基于PCR-RFLP梨品种cpDNA遗传多态性的分析

Genetic Diversity of Nineteen Cultivars in Pyrus Derived from cpDNA PCR-RFLP

为了研究库尔勒香梨等19个主要梨品种的cpDNA遗传多态性,采用PCR-RFLP进行分析,利用10对通用引物对总DNA进行扩增,并且采用7种限制性内切酶对PCR产物进行酶切,应用DPS v7.05版软件计算Jaccard相异系数,采用非加权类平均法(UPGMA)进行0-1系统聚类分析。结果显示:7对引物(cp01、cp02、cp03、cp04、cp06、cp09、cp10)能在梨属植物上扩增出1条特异性谱带,cp09/MvaI,cp03/Hin6I的酶切位点有显著差异。根据结果分析,库尔勒香梨与鸭梨、砀山梨、苹果梨、早酥、慈梨、金川雪梨、锦丰、新疆句句梨的平均距离系数较小,与其他梨的平均距离系数较大。

This experiment researches into the genetic diversity of nineteen cultivars in Pyrus derived from cpDNA PCR-RFLP. Ten universal primer pairs of chloroplast genome were used to amplify cpDNA noncoding regions in nineteen Pyrus species. The PCR products were digested by seven restriction enzymes. Seven primer pairs （cp01, cp02, cp03, cp04, cp06, cp09, cp10） generated special bands from Pyrus. The fragements digested of cp09/MvaI,cp03/Hin6I were different. DPS v7.05 was used to calculate Jaccard, and the dendrogram was constructed by UPGMA. The average distance coefficient of the Korla pear with Yali, Dangshanli, Pingguoli, Cili, Jinchuanxueli, Jinfeng and Juj uli were smaller; that of Korla pear with the other cultivars were bigger.