Tempol对UVB所致HaCaT细胞凋亡的抑制作用

Inhibitory effects of Tempol on HaCaT cells apoptosis caused by UVB

目的:确定4-羟基-2,2,6,6-四甲基氧基哌啶(Tempol)对UVB所致HaCaT细胞凋亡的抑制作用。方法:HaCaT细胞分7组,A组:对照组;B组:UVB辐照组;C组:0.5 mM Tempol实验组;D组:1 mM Tempol实验组;E组:2 mM Tempol实验组;F组:4 mM Tempol实验组;G组:8 mM Tempol实验组。除A组外,其余组均辐照UVB,分析各组间HaCaT细胞增殖效应及细胞凋亡率差别。结果:与B和G组比较,各组HaCaT细胞增殖效应显著升高(P<0.01);与A组比较,(除B组外),各组HaCaT细胞凋亡率随Tempol药物浓度的升高而显著升高(P<0.01)。结论:Tempol对UVB所致HaCaT细胞凋亡有抑制作用,一定范围内其作用随Tempol浓度的升高而降低。

Objective： To determine the inhibitory effect of 4- hydroxy - 2,2,6,6- tetramethyl- piperidine 1 - oxyl （Tempol） on apoptosis caused by UVB irradiation in HaCaT cells. Methods： HaCaT cells were divided into seven groups： group A： control group, group B： UVB radiation group, group C： supplied with 0.5 mM Tempol, group D： supplied with 1 mM Tempol, group E： supplied with 2 mM, group F： supplied with 4 mM Tempol and group G： supplied with 8 mM Tempol. Except for group A, other groups were radiated with UVB, and the different cell proliferation and the apoptosis between each group were analyzed. Results： Compared with group B and G, the cell proliferation in each group was elevated significantly （ P 〈 0.01 ） ; and compared with group A, the apoptosis rate in each group was elevated significantly as the concentration of Tempol is increased except for group B （ P 〈 0.01）. Conclusion： Tempol has an inhibitory effect on photodamage caused by UVB in HaCaT cells, in which the effect is decreased as the concentration of Tempol is increased in a certain range.