甘草等提取物对UVB诱导小鼠皮肤HSP27基因表达的影响

Effect of extractive of glycyrrhiza AM and rhodiola rosea L on the expression of HSP27 mRNA in skin of BALB/c mice irradiated by ultraviolet B

目的:评价甘草、红景天、黄芪提取物对中波紫外线(UVB)照射诱导BALB/c小鼠皮肤HSP27mRNA表达水平的影响。方法:分组将自制5%与10%甘草、红景天、黄芪提取物溶液涂于小鼠背部皮肤,20min后给予500mJ/cm^2 UVB照射,每日1次,连续30天;设UVB对照组与空白对照组。RTPCR法检测各组小鼠背部皮肤组织中HSP27 mRNA的表达水平。结果:UVB照射组小鼠皮肤中HSP27 mRNA表达水平较空白对照组显著增高(P<0.05),不同剂量甘草、红景天、黄芪处理后可进一步上调HSP27表达水平(P<0.05)。结论:UVB照射可诱导小鼠皮肤HSP27 mRNA表达增加,甘草、红景天、黄芪可进一步诱导HSP27 mRNA表达增加。

Objective： To investigate the effects of topical application of extractive of glycyrrhiza, astrafalus membrailaceus （AM）, and rhodiola rosea L on the expression of HSP27 mRNA in the skin of BALB/c mice irradiated by ultraviolet B （UVB）. Methods： 5% and 10% glycyrrhiza, rhodiola rosea L, AM lutions were topically used on BALB/c mice back, which 20 min later, were irradiated by UVB 500 mJ/cm： once a day for consecutive 30 d （treatment group）. The skin samples were obtained after one month and the expression of HSP27 mRNA was measured by RT- PCR. The results were compared with the mice treated with UVB irradiation only （UVB irradiation group）. The mice without any treatment were used as blank control. Results： Compared with blank control group, the expression of HSP27 mRNA in UWB irradiation group was significantly increased （ P 〈 0.05 ）. Conclusion： Chronic UVB irradiation can upregulate the expression of HSP27 mRNA, which is enhanced by topical application of glycyrrhiza, AM, rhodiola rosea L in the skin of BALB/c mice.