摘要
目的通过实时荧光定量PCR法测定孕妇血浆中胎儿表观遗传学标记物非甲基化丝氨酸蛋白酶抑制剂B5(un-methylated serpin peptidase inhibitor,clade B,member 5,U-maspin)基因的含量,初步探讨母血浆中U-maspin基因的定量能否预测子痫的发生.方法随机选择子痫前期孕妇40例为实验组,其中轻度和重度患者各20例,以健康同期妊娠妇女20例为对照组.提取血浆游离DNA,用甲基化特异性PCR(MSP)方法检测各组血浆标本中U-maspin基因的含量.结果子痫前期组孕妇血浆中U-maspin的水平高于对照组,差异有统计学意义(P<0.05),轻、重度子痫前期患者平均浓度有差异,分别为1 402.05 copies/mL及2 189.60 copies/mL(P<0.05).结论U-maspin基因作为孕妇血浆中游离的胎儿特异性表观遗传学标记物,可以预测子痫的发生.
Objective Apply Fluorescence quantitative polymerase chain reaction (FQ-PCR) to quantify un-methylated serpin peptidase inhibitor, clade B (un-methylated maspin) gene in maternal plasma, and to study if it can be used to predict eclampsia. Methodsd Forty pre-eclamptic pregnant women including mild pre-eclampsia(20 cases) and severe pre-eclampsia (20 cases) were selected as study guoup, twenty gestational age-matched normal pregnancies were selected as control group. Free DNA of plasma samples was extracted, The methylation-specific PCR (MSP) method was used to detect the expression of U-maspin gene. Results The level of fetal DNA in preeclampsia was higher than that of controls (P〈0.05). There was significant difference between mild and severe pre-eclampsia subjects, The mean concentrations were 1 402.05 copies/mL and 2189.60copies/mL (P〈0.05) respectively. U-maspin gene may be considered as a epigenetic marker to detect the fetal DNA in maternal plasma, and it maybe helpful to predict eclampsia.
出处
《山西大同大学学报(自然科学版)》
2010年第2期44-47,共4页
Journal of Shanxi Datong University(Natural Science Edition)
基金
国家人口计生委资助项目[2005]11号C1-41
山西省科技厅攻关项目[NO.20080311072]