松木层孔菌多糖PEP的结构及活性研究

The study of structure and immunobiological activity of the polysaccharide from the mycelium of Phellinus pini

从松木层孔菌发酵菌丝体中分离制备碱提水溶性多糖(PEP).高效液相色谱分析表明,PEP为单一级分,相对分子质量约为1.6×104.GC分析PEP的单糖组成为甘露糖、半乳糖和葡萄糖,其物质的量比为3.11∶1.32∶0.54.PEP经部分酸水解、高碘酸氧化、Smith降解和甲基化分析,其结构主要为β-(1→2)Man且在6-0处构成分枝结构,PEP的支链部分由(1→4)-Man,(1→6)-Gal构成,末端残基为Gal和Glc.初步考察,PEP对小鼠离体淋巴细胞有较显著的促增殖作用.

The mycelial polysaccharides（PEP）were extracted from fermental mycelial of Phellinus pini using alkali.HPLC analysis manifested that it（PEP） was uni-form fraction and the molecular weight was about 16 thousand,GC analysis indicated that the PEP were composed of Man,Gal,and Glc with the molar ratio of 3.11∶1.32∶0.54.After the methods of partial hydrolsys with acid,periodate oxidation,Smith degradation,methylation and GC-MS analysis,the results indicate that the main chain of PEP is mainly made up of β-（1 →2） Man and substituted at 6-0.The side chain is composed of（1→4）-Man and（1→6）-Gal,the nonreduced end is composed of Gal and Glc.Pharmaceutical experiments showed PEP have a significant effect on the immunomodulatory activities of murine splenic lymphocytes in vitro.