摘要
从松木层孔菌发酵菌丝体中分离制备碱提水溶性多糖(PEP).高效液相色谱分析表明,PEP为单一级分,相对分子质量约为1.6×104.GC分析PEP的单糖组成为甘露糖、半乳糖和葡萄糖,其物质的量比为3.11∶1.32∶0.54.PEP经部分酸水解、高碘酸氧化、Smith降解和甲基化分析,其结构主要为β-(1→2)Man且在6-0处构成分枝结构,PEP的支链部分由(1→4)-Man,(1→6)-Gal构成,末端残基为Gal和Glc.初步考察,PEP对小鼠离体淋巴细胞有较显著的促增殖作用.
The mycelial polysaccharides(PEP)were extracted from fermental mycelial of Phellinus pini using alkali.HPLC analysis manifested that it(PEP) was uni-form fraction and the molecular weight was about 16 thousand,GC analysis indicated that the PEP were composed of Man,Gal,and Glc with the molar ratio of 3.11∶1.32∶0.54.After the methods of partial hydrolsys with acid,periodate oxidation,Smith degradation,methylation and GC-MS analysis,the results indicate that the main chain of PEP is mainly made up of β-(1 →2) Man and substituted at 6-0.The side chain is composed of(1→4)-Man and(1→6)-Gal,the nonreduced end is composed of Gal and Glc.Pharmaceutical experiments showed PEP have a significant effect on the immunomodulatory activities of murine splenic lymphocytes in vitro.
出处
《分子科学学报》
CAS
CSCD
北大核心
2010年第2期103-107,共5页
Journal of Molecular Science
基金
吉林省科技厅资助项目(20060559)
关键词
松木层孔菌
多糖
结构分析
淋巴细胞转化
Phellinus pini
polysaccharide
constructure analysis
splenocyte pro-liferation