骨髓基质细胞不同培养方法效果比较

A comparative study of differenation culture method for rabbit marrow stroma cell

目的:观察全骨髓培养法与密度梯度离心法对培养骨髓基质细胞的效果差异,建立简单、经济、实用分离骨髓间充质干细胞方法。方法:体外提取骨髓基质细胞,分别采用全骨髓培养法与密度梯度离心法培养骨髓基质细胞,观察细胞形态学、细胞活力、细胞周期及细胞表面分子表达差异。结果:两种方法均能有效培养出骨髓间充质干细胞,各细胞周期的细胞检出率无显著差异(P>0.05),细胞形态学无差异,细胞表面分子CD34、SH1、SH2、SH3表达差异不显著(P>0.05)。结论:全骨髓培养法在分离培养骨髓基质细胞方面与密度梯度离心法具有类似效果。

Objective.. To comparative differenation culture method for rabbit marrow stroma cell （MSC）. Methods： Isolated MSCs from 24 rabbits bone marrow were cultured in cell culture medium with density gradient ultracentrifugation method and all cellulae medullares method respectively. Results： The cell morphology, cell number of each cycle and cell surface molecule expression no significantly difference between two method （P〉0.05 all）. Conclusion： Compared with density gradient ultracentrifugation method, the all cellulae medullares method is still an effective method.