氯沙坦对高脂血症大鼠血小板功能的影响

Intervention effect of losartan on the platelet function of hyperlipemia rats

目的:分析高脂血症大鼠血小板功能的变化,探讨氯沙坦对高脂血症大鼠血小板功能的影响。方法:34只Wistar大鼠被随机分为正常对照组、高脂血症模型组、氯沙坦组(科素亚,20 mg/kg.d)组。分组进行干预后检测3组大鼠的血脂:总胆固醇(TC)、甘油三酯(TG)、低密度脂蛋白胆固醇(LDL-C)、高密度脂蛋白胆固醇(HDL-C)的水平。应用酶联免疫法测定血浆中血小板颗粒膜蛋白(GMP140)浓度、流式细胞术检测血小板表面CD62P的表达。结果:分组干预4周后,高脂血症组和氯沙坦组的TC[(13.779±5.219)mmol/L,(14.570±8.357)mmol/L],LDL-C[(10.680±4.727)mmol/L,(11.393±7.623)mmol/L]含量显著高于正常对照组[(1.846±0.196)mmol/L,(0.164±0.031)mmol/L],P均<0.001,HDL-C/TC[(0.195±0.079)mmol/L,(0.216±0.053)mmol/L]含量显著低于正常对照组(0.583+0.062)mmol/L,P<0.001;高脂血症组和氯沙坦组的血脂含量无显著差异(P均>0.05)。高脂血症组和氯沙坦组的GMP140[(27.045±2.849)ng/ml,(20.665±1.701)ng/ml]、CD62P[(25.169±5.324)%,(20.144±1.290)%]含量显著高于正常对照组的[(10.655±1.682)ng/ml,(10.089±1.333)%],P<0.001。氯沙坦组GMP140及CD62P水平均显著低于高脂血症组(P<0.05)。结论:高脂血症能够促使血小板活化,氯沙坦具有抑制血小板活化的作用。

Objective： To analyze the platelet/unction change of hyperlipemia rats and evaluate the intervention effect of losartan on the platelet function of them. Methods： Thirty four Wistar rats were randomly divided into three groups： control group, hyperlipidemia group and losartan group （Kesuya, 20 mg/kg/d）. After four weeks of intervention, the serum levels of lipid （include TC, TG, LDL-C and HDL-C） were measured. Euzymelinked immunosorbent assay was used to detect the serum levels of GMP140. Flow cytometric analysis was applied to detect the expression of CD62 on the surface of platelet. Results： Four weeks after intervention, the levels of TC [ （13. 779±5. 219） mmol/L, （14. 570±8. 357） mmol/L], LDL-C [ （10. 680±4. 727） retool/L, （11. 393±7. 623） mmol/L] in hyperlipidemia group and losartan group were significantly more than those of control group [ （1. 846±0. 196） mmol/L, （0. 164± 0. 031） mmol/L], P〈0. 001 all, the level of HDL-C/TC [ （0. 195±0. 079） mmol/L, （0. 216±0. 053） mmol/L] in hyperlipidemia group and losartan group were significantly less than that of control group, P〈0. 001. The levels of lipid above stated between hyperlipidemia group and losartan group were no significant difference （P〉0.05 all）. The levels of GMP140 [ （27. 045±2. 849） ng/ml, （20. 665±1. 701） ng/ml], CD62P [ （25. 169±5. 324）%, （20. 144±1. 290）%] in hyperlipidemia group and Iosartan group were significantly more than those of control group [ （10. 655± 1. 682） ng/ml, （10. 089±1. 333））%], P〈0. 001. The levels of GMP140, CD62P in Iosartan group was significantly less than those of hyperlipidemia group （P〈0.05）. Conclusion.. Hyperlipidemia can enhance platelet activation. Losartan can improve platelet function and refrain thc activation of platelet.