大豆蛋白质含量的QTL定位

QTLs Mapping Related to Protein Content of Soybeans

以高蛋白的大豆品种齐黄26和低蛋白的滑皮豆为亲本,杂交获得含170个单株的F2代分离群体,采用SSR分子标记技术,构建了一张包括18个连锁群的分子连锁图谱,覆盖大豆基因组长度1 035.6 cM,标记间平均距离为16.44 cM。利用复合区间作图法,对在济南和冠县衍生出的F2∶3群体的蛋白质含量的进行QTL分析。结果表明:济南试验点定位到3个与蛋白质含量有关的QTL,分布于D2、E和K连锁群上,分别可解释14%、11%和2%的变异;冠县试验点定位到1个与蛋白质含量有关的QTL,位于E连锁群上,可解释3%的变异。通过遗传作图找到3个与所定位的QTL相连锁的SSR标记,这些标记可为大豆分子标记辅助育种提供参考。

Soybean is a versatile crop due to its multitude uses as a high protein meal and vegetable oil. Seed protein content is an important quantitative traits. To identify the quantitative traits loci （QTL） related to seed protein content,a cross was made with the high protein content variety Qihuang 26 and the low protein content germplasm Huapidou, and derived a F2 population with 170 plants. Two F2：3 populations were derived from the F2 population in Jinan and Guanxian respectively. SSR marker data of F2 population and phenotype data of F2：3 population were analyzed with Mapmaker/EXP V3.0 and QTL Cartographer 2.5. One molecular genetic linkage map with 18 linkage groups （LGs） and covering 1 035.6 cM was mapped and 3 QTLs related to seed protein content were identified on D2, E and K linkage groups, explaining 14% , 11% and 2% phenotypic variance respectively in Jinan, and 1 QTL related to seed protein content was identified on E linkage group in Guanxian, explaining 3% phenotypic variance.