蚜虫取食和机械损伤对大豆真叶中异黄酮的诱导作用

Comparing on the Inducing Isoflavonoids Content in Leaf of Soybean Aroused by Soybean Aphid and Mechanical Damage

采用高效液相色谱法,通过测定大豆真叶异黄酮含量的变化,探讨大豆蚜虫取食和机械损伤与大豆真叶异黄酮含量之间的化学诱导关系。结果表明:蚜虫取食诱导的异黄酮合成反应速度较快,而机械损伤处理诱导的异黄酮合成反应速度相对较慢;蚜虫取食引起大豆真叶内大豆黄素和染料木素苷元含量的同时增加,而机械损伤处理提高黄豆黄素苷元含量却降低了大豆黄素苷元含量;蚜虫取食能在相当长的时间内维持异黄酮含量的增加,而机械损伤处理只能在短时间内引起异黄酮含量的变化。因此,蚜虫取食和机械损伤处理所诱导的异黄酮含量变化的规律不同,蚜虫取食和机械损伤所诱导的大豆抗性不是完全对等的。

Experiments were conducted to determine whether the content of isoflavonoids is increased by the damage caused by soybean aphid and machines. Samples from leaf of soybean damaged by soybean aphid and machines were analyzed for isoflavonoids content with High Performance Liquid Chromatography（HPLC）, Compared with mechanical damage, soybean aphid infection increased the synthetic velocity of isoflavonoids, hence, increased the daidzin and genistin content. Mechanical damage increased glycitin while reduced daidzin content. The duration of induction reaction in ahpid-infested soybean persisted much longer than that of the mechanically damaged plants. Results suggest the changing trends of isoflavonoids content and the induced resistance resulted form the soybean aphid and the mechanical damage were quite different.