白唇竹叶青蛇毒5′-核苷酸酶对血小板聚集的影响及其机制研究

The effect of 5'-nucleotidase from Trimeresurus albolabris venom on platelet aggregation and its mechanism

目的研究白唇竹叶青蛇毒5′-核苷酸酶对血小板聚集的影响,探讨其作用机制。方法采用比浊法测定白唇竹叶青蛇毒5′-核甘酸酶对二磷酸腺苷(ADP)、花生四烯酸(AA)、血小板活化因子(PAF)诱导血小板聚集的影响。结果白唇竹叶青蛇毒5′-核甘酸酶能抑制ADP、AA、PAF诱导的血小板聚集,且其抑制率与剂量增加成正比;抑制血小板聚集作用与ADP的水解和腺苷的积累有关。结论白唇竹叶青蛇毒5′-核甘酸酶能抑制血小板聚集,抑制作用主要通过水解ADP和积累腺苷,可能与阻断TXA2的生成有关。

Purpose To investigate the effect of 5＇-nucleotidase from Trimeresurus albolabris venom on platelet aggregations and its mechanism.Methods Platelet aggregations induced by ADP,AA and PAF,respectively,were measured by turbidimetric method after platelet incubated with 5＇-nucleotidase.Results 5＇-nucleotidase significantly inhibited platelet aggregations induced by ADP,AA and PAF in a dose-dependent manner. Washed platelet inhibition experiment and the effect of Adenosine,CP/CPK on platelet aggregations showed that platelet aggregations inhibited by 5＇-nucleotidase were associated with ADP hydrolysis and adenosine accumulation. The effect of ASA on platelet aggregations showed that the enzyme inhibited platelet aggregations probably by blocking the formation of TXA2.Conclusion 5＇-nucleotidase from Trimeresurus albolabris venom inhibited platelet aggregations through ADP hydrolysis and adenosine accumulation,and maybe it is related to blocking the formation of TXA2.