马尾松花粉多糖硫酸酯化前后生物活性的比较研究

Comparative study on bio-activity of polysaccharide from Pinus massoniana pollen and its sulfated derivative

目的对马尾松花粉多糖(PPM)进行硫酸酯化,得硫酸酯化多糖(S-PPM),比较酯化前后抗肿瘤活性、免疫调节活性和清除自由基能力的变化。方法PPM采用氯磺酸-吡啶法进行硫酸酯化。结果S-PPM取代度为1.47,S-PPM对体内外肿瘤细胞的抑制作用、对促进T、B细胞增殖作用均高于PPM;对促进巨噬细胞的吞噬作用变化不大;S-PPM清除超氧阴离子自由基的作用明显强于PPM,清除羟自由基的作用小于PPM。结论S-PPM能促进特异性免疫抑制肿瘤细胞的增殖;硫酸酯化也能改善对多糖清除自由基的作用。

Purpose To study the polysaccharide from Pinus massoniana pollen （PPM） and to compare its anti-tumor,immune modulation activities and scavenging qualities of free radical with its sulfated derivative （S-PPM）.Methods PPM was chemically modified by chlorosulfonic acid-pyridine method and their bioactivities were compared.Results The substituting degree of S-PPM was 1.47.Results showed that S-PPM was more powerful in inhibiting the growth of tumor cell in vivo and in vitro and in promoting T,B lymphocytes than PPM.But there was no remarkable difference in promoting phagocytosis of macrophages.S-PPM was stronger in scavenging superoxide anion radical than PPM but it was vice versa in scavenging hydroxyl radical.Conclusion S-PPM inhibited the cancer cell growth mainly through specific immunity.Sulfate of PPM influenced its quality of scavenging free radicals greatly.