分光光度法测定海水中叶绿素含量的研究

Detection the Content of Chlorophyll in Seawater by Spectrophotometric Method

近年来,海洋污染日益成为世界各国严重关切的问题。叶绿素a是环保部门监测水质状况的重要指标之一,可通过海水中叶绿素a浓度来估算海洋初级生产力。文章系统比较了三种常用有机萃取溶剂:90%丙酮、90%热乙醇,N,N-二甲基甲酰胺(DMF)以及不同萃取时间对叶绿素a的萃取效果,结果显示DMF做萃取剂萃取较为完全,且所用时间短,其中测定的变异系数RSD-DMF为2.9%,满足叶绿素a含量检测的要求。

Marine pollution became a global problem that all the countries were deeply concerned about in recent years. So chlorophyll a （Chla） was one of the important indicators of water quality monitored by the department of environmental protection. In addition, the content of Chla in seawater can also be taken as the estimation for the elementary productivity of ocean. By comparing with the 90 % acetone, 90 % ethanol and N, N - dimethylformamide （ DMF）, and the effect of extraitiom of chlorophyll a （Chla） at different times, the results of experiments indicated that the N,N -dimethylformamide （DMF） had an excellent extraction effect, coefficient of variability of DMF was 2. 9 % , and satisfied the requirement of the detection of chlorophyll content.