还原型谷胱甘肽对体外循环期细胞因子IL-8和IL-6的影响

The effects of GSH on Cytokines IL-8,IL-6 during cardiopulmonary bypass

目的观察还原型谷胱甘肽对体外循环(CPB)期间白细胞介素-8(IL-8)和白细胞介素-6(IL-6)的影响。方法选择30例施行心脏手术患者,随机分成实验组和对照组(每组15例)。实验组患者于手术开始时分别予还原型谷胱甘肽(35 mg/kg)溶于100 ml生理盐水静滴,对照组予生理盐水静滴。于CPB前(T1)、CPB 30 min(T2)、CPB停机(T3)、停机后2 h(T4)四个时点采血,采用放射免疫法测定两组血清中IL-8和IL-6的浓度。结果实验组IL-8、IL-6血清浓度较对照组降低,差异有统计学意义(P<0.05)。结论还原型谷胱甘肽能减少CPB期间IL-8和IL-6的释放,减轻全身炎性反应的程度。

Objective To investigate the effects of glutathione （GSH） on interleukin-8（IL-8） and interleukin-6 （IL-6） during cardiopulmonary bypass（CPB）. Methods Thirty patients undergoing Cardiac surgery were randomly divided into GSH group and control group（15 in each group）. In GSH group,intravenous drip of 100 ml of GSH（35 mg/kg）was given at the beginning of surgery. Respectively, while the control group was given 0.90/00 NaCl solution. Blood samples were taken before CPB,30min after CPB and 0h,2h after the end of CPB. The serum levels of IL-8 and IL-6 were measured by radioimmunoassay method in both groups. Results The serum levels of IL-8 and IL-6 in GSH group were significantly lower than that in control group（ P 〈0.05）. Conclusion The study shows that GSH could reduce the release of IL-8,IL-6 and inhibit systemic inflammatory response syndrome（SIRS） during CPB.