摘要
利用X射线对体外培养的人肝癌细胞SMMC-7721和正常人肝细胞HL-7702进行辐照,以细胞克隆形成法测定细胞存活率,多聚酶链式反应银染端粒重复序列扩增法(Telomeric repeat amplification protocol,TRAP-PCR)检测细胞中端粒酶活性变化,探讨辐照后细胞存活与细胞中端粒酶活性变化的关系。结果表明,SMMC-7721和HL-7702细胞的存活率都随辐照剂量的增加而相应降低。在1-4Gy剂量范围内,两种细胞的端粒酶活性变化均呈剂量依赖性增强。但在1Gy、2Gy、3Gy剂量下,SMMC-7721其端粒酶活性较对照(0Gy)细胞低;当辐照剂量达4Gy时,端粒酶活性略高于对照细胞。在1-4Gy剂量范围内,HL-7702和SMMC-7721的细胞存活与端粒酶活性在变化趋势上呈负的关系。
In this paper,the relationship between cell survival fraction and telomerase activity was investigated with the human hepatocellular cells HL-7702 and carcinoma cells SMMC-7721 irradiated by X-rays. The colony formation assay and TRAP （Telomeric repeat amplification protocol） method were used to determine the cell survival fraction and telomerase activity respectively. To both of HL-7702 and SMMC-7721,the telomerase activity was increased in dose-dependent. However,at 1—3 Gy,the telomerase activity was lower than that of control in SMMC-7721,and it was a little higher than that of control when irradiated at 4 Gy. At 1—4 Gy,the variation trend of cell survive and that of telomerase activity were identical.
出处
《辐射研究与辐射工艺学报》
CAS
CSCD
2010年第2期119-123,共5页
Journal of Radiation Research and Radiation Processing
