苦荞芽中黄酮类化合物的抑菌作用研究

Studies on Antibacterial activity of Flavonoids from Germination of Buckwheat

采用分光光度法测定萌发7d内的苦荞芽中苯丙氨酸解氨酶(PAL)比活力与黄酮类化合物的含量变化关系;滤纸片扩散法测定苦荞芽提取物对大肠杆菌、金黄色葡萄球菌、枯草芽孢杆菌和沙门氏菌的抑制效果。结果表明:荞麦萌发3d后,PAL比活力与黄酮类化合物的含量随着萌发天数的增加而上升,且PAL比活力与黄酮类化合物的含量呈同步变化关系。苦荞芽提取物对大肠杆菌、金黄色葡萄球菌、枯草芽孢杆菌和沙门氏菌均具有抑制效果,其中对沙门氏菌的抑菌效果最为显著。

The specific activity of PAL and the content of flavonoids were investigated via spectrophotography and the inhibitory effect of the extractive on E. coli, S. aureus, B. subtilis and S.typhimurium were detected via filter paper diffusion method in buckwheat germinated from 0d to 7d. The results showed that the activity of PAL and the content of flavonoids grow in step with the germinating days during the 3 d, they keep in the certain concentration range in the later days of germinated buckwheat. There is different inhibitory effect on E. coli, S. aureus, B. subtilis and S.typhimurium of the germination of buckwheat, the antibacterial activity against S.typhimurium was the most obvious.