苦瓜提取液的抑菌谱及其稳定性研究

Research on Antibacterial Spectrum and Stability of Extracting Solution from Momordica Charantia L.

以抑菌效果为评价指标,采用革兰氏阳性菌和阴性菌中最具代表性的菌种作为指示菌,研究苦瓜粗提液中抑菌活性成分的抑菌谱及其相关的稳定性。试验结果表明:苦瓜粗提液对细菌有较强的抑制作用。其中,苦瓜提取液对大肠杆菌的最低抑菌浓度(MIC)低达18.75mg/mL,而其对枯草杆菌的MIC更是低达9.375mg/mL。但其对霉菌和酵母菌没有明显抑制作用。在同等浓度下,苦瓜提取液对细菌的抑制作用强于山梨酸钾。同时,也表明该提取液在一定范围的温度、pH环境、紫外光照射时间和无机盐浓度的条件下仍然能保持较好的抑菌活性。

Antibacterial spectrum and stability of extracting solution from momordica charantia L. were studied in this paper. The result show that bacterias like escherichia coli and bacillus subtilis are inhibited by the extracting solution well, and the MIC are 18.75 mg/mL and 9.375 mg/mL. However, mold and yeast aren＇t controlled obviously. The inhibiting effect of the extracting solution is better than potassium sorbate under the same concentration. Meanwhile, the stability of the extracting solution is well under certain temperature, pH, UV exposure time and concentration of inorganic salts.