喉鳞癌中HHV6、HPV16/18的表达及其相互关系

Expression of human herpesvirus 6 and human papillomavirus 16/18 in the laryngeal squamous cell carcinoma and their relations

目的分析喉癌组织中人类疱疹病毒6(human herpesvirus6,HHV6)与人乳头状瘤病毒16/18(human papillomavirus16/18,HPV16/18)的表达及其相互关系。方法用免疫组化SP法和逆转录聚合酶链式反应(reverse transcription polymer-ase chain reaction,RT-PCR)及巢式RT-PCR方法测定HPV16/18、HHV6在喉鳞癌组织、癌旁正常组织及声带息肉组织中的表达。结果HPV16/18,HHV6在喉鳞癌组织、癌旁正常组织和声带息肉组织之间表达存在明显差异。②HPV16/18表达在喉癌不同病理分化程度之间有差异,分化程度低者,表达量高;HHV6表达在喉鳞癌不同分化程度间均未发现显著性差异,无明显相关性。③HHV6和HPV16/18在喉癌组织中表达呈高度一致性,有相关关系。结论HHV6可能是喉鳞癌的致病因子,并作为促进子直接参与和/或通过上调HPV16/18协同促使喉鳞癌进展。

Objective To explore the relationship between the expression of human herpesvirus 6（HHV6）and human papillomavirus 16/18（HPV16/18）in laryngeal squamous cell carcinoma.Methods HPV16/18 expression and HHV6 expression were detected by immunohistochemistry and nested-reverse transcription polymerase chain reaction（RT-PCR）in the tissues of laryngeal squamous cell carcinoma,adjacent normal tissues and tissues of vocal cord polyp.Results ①The expression of HPV16/18 and HHV6 showed significant difference between the tissues of laryngeal squamous cell carcinoma,tissues of vocal cord polyp,and adjacent normal tissues.②HPV16/18 expression manifested a significant difference in different grades of differentiation.The lower the degree of differentiation was,the higher the expression was.There was no significant association between HHV6 and the differentiation grade of laryngeal squamous cell carcinoma.③The expression of HHV6 and HPV16/18 showed a high concordance in laryngeal squamous cell carcinoma,and there was a significant correlation between them.Conclusion HHV6 may be a causative agent of laryngeal squamous cell carcinoma.In addition,as a promoter,it may participate in and/or up-regulate HPV16/18 expression for urging the development of laryngeal squamous cell carcinoma.