黄芪对尾加压素Ⅱ诱导的心脏成纤维细胞胶原合成及分泌转化生长因子-β_1的影响

The effect of astragalus membranaous on urotensinⅡ-induced collagen synthesis and transforming growth factor-β1 scretion of cardiac fibroblasts

目的观察黄芪注射液对尾加压素Ⅱ(UⅡ)诱导的心脏成纤维细胞合成胶原及分泌转化生长因子(TGF-β1)的影响。方法体外培养乳鼠心脏成纤维细胞,分成3组:对照组、UⅡ组、UⅡ+黄芪组。作用一定时间后,Real-time RT-PCR法测定各组细胞Ⅰ、Ⅲ型胶原及TGF-β1基因表达情况,3H-脯氨酸掺入测定胶原合成情况,双抗体夹心ELISA法测定培养上清TGF-β1分泌情况。结果UⅡ可以促进乳鼠心脏成纤维细胞胶原合成,刺激TGF-β1的分泌,黄芪注射液可明显抑制UⅡ的上述作用。结论黄芪可以通过抑制UⅡ诱导的心脏成纤维细胞胶原合成及TGF-β1分泌,延缓心肌纤维化及心脏重构的进展。

Objective To observe the effect of astragalus membranaous on UrotensinⅡ（UⅡ）-induced collagen synthesis and transforming growth factor-β1 scretion of cardiac fibroblasts.Methods The neonatal cardiac fibroblasts were cultured and the forth generation cells were allocated into 3 groups：control group （cells were cultured under normal conditions）,UⅡ groups（cells were cultured with 10～7mol/L UⅡ）,astragalus membranaous groups（cells were cultured with 10～7mol/L UⅡ and 40mg/L astragalus membranaous）respectively.After certain periods of time,the mRNA expression of collagenⅠ,collagen Ⅲ and TGF-β1 were measured by real-time RT-PCR,collagen synthesis was measured by 3H-proline incorporation and TGF-β1 secretion was measured by ELISA.Results UⅡ significantly induced collagen synthesis and secretion of TGF-β1 in neonatal cardiac fibroblasts in vitro and astragalus membranous could reduce these effects of UⅡ.Conclusion Astragalus membranaous can delay the progression of cardiac fibrosis and cardiac remodeling by inhibiting the UⅡ-induced collagen synthesis and TGF-β1 secretion in cardiac fibroblasts.