高压脉冲电场静态处理室的研制

Developing on the Static Chamber of High-Voltage Pulsed Electric Field

高压脉冲电场（Pulsed Electric Field,PEF）是一项极具前景的食品非热杀菌技术,其处理关键部件——处理室直接影响杀菌效果。作者设计了电场分布均匀、处理物料容量可调、安全可靠的PEF处理室,应用该PEF处理室对接种大肠杆菌的液蛋进行杀菌,当电场强度为34.2 kV/cm处理时间为400μs时,原样品菌体浓度为10^6-10^7cfu/mL,大肠杆菌细菌数量降低了约5.05个对数,从而说明作者设计的静态处理室是可行的,可以进行杀菌钝酶等相关机理研究。

High-voltage Pulsed Electric Field（PEF） is a very promising non-thermal food sterilization technology.The key component,PEF chamber,directly influences the sterilization effect.In this study,a safe and reliable PEF chamber which has a uniform distribution of the electric field and an adjustable capacity of handling materials was designed.The static chamber designed was applied in the pulsed electric fields（PEF） to sterilize the inoculated E.coli in Liquid Whole Egg（LWE）.In the original samples of the bacteria whose concentration of E.coli bacteria is 106-107cfu /ml,5.05 log Sterilization was achieved by using PEF-only processing at 34.2 kV/cm for 400μs.This result shows that the designed static treatment room is feasible,and it can be used in the related mechanism research,such as sterilization and inactivating enzyme.