中药材香圆挥发油成分GC-MS分析与比较

Comparison of Volatile Oils from both Fructus Aurantii and Fructus Aurantii Immaturus of Citrus wilsonii in Shaanxi by GC-MS

为确立陕西地产枳实与枳壳的药用地位,以陕西汉中地产香圆(Citrus wilsonii Tanaka)的枳实、枳壳为材料,采用水蒸汽蒸馏法提取其中的挥发油,用GC-MS进行定性、定量分析,并与基原酸橙(C.aurantium)枳实与枳壳中的挥发油成分进行比较。结果表明,香圆枳实中挥发油已知成分计65种,占挥发油总质量的98.91%,主要成分有D-柠檬烯、γ-萜品烯、β-荜茄澄烯和α-蒎烯等;枳壳中挥发油已知成分计75种,占挥发油总质量的99.89%,主要包括D-柠檬烯、γ-萜品烯、β-香叶烯和α-松油醇等;两者有相同成分44种。与基原酸橙枳实与枳壳挥发油成分相比,香圆枳实含相同成分8种,差异成分57种;枳壳挥发油中相同成分有12种,差异成分63种;其柠檬烯和萜品烯的含量均高于基原酸橙。因此,香圆枳实、枳壳挥发油成分与规定基原酸橙等存在明显差异。

The volatile oils origin from both Fructus Aurantii Immaturus（FAI） and Fructus Aurantii（FA） of Citrus wilsonii Tanaka grown in Hanzhong,Shaanxi Province were extracted by steam distillization,and qualitatively and quantitively analyzed by GC-MS in this study.The differences between C.wilsonii and C.aurantium on volatile oils were also compared.The FAI of C.wilsonii were composed of 65 volatile oils,with the main components of D-limonene,γ-terpene,β-cubebene and α-pinene.FA contained 75,with D-limonene,γ-terpene,β-myrcene and α-terpineol as the main components.A total of 44 common constituents existed in both FAI and FA of C.wilsonii.Compared with volatile oils of C.aurantium,those in FAI of C.wilsonii were 8 similar and 57 different,and those in FA share 12 same substrance.Therefore,volatile oils were very similar in both parts of the same species,however,there are different between C.wilsonii and C.aurantium.