莪术油中莪术醇 莪术二酮在大鼠体内的组织分布研究

Investigation of the Tissue Distribution of Zedoary Oilin Rats by GC

目的：研究莪术油的在大鼠体内的分布特点。方法：采取大鼠灌胃给药,运用毛细管气相色谱法以α-香附酮为内标物同时测定莪术油中指标性成分莪术醇、莪术二酮在大鼠各个组织中的药物浓度。结果：莪术醇在0.002628-0.2102mg.mL^-1范围内呈现良好的线性关系,莪术二酮在0.0004552-0.03642mg.mL^-1范围内呈现良好的线性关系,精密度、稳定性及融冻次数考察均符合要求,测定方法的平均绝对回收率莪术醇为75.8-79.8%,莪术二酮为82.9%-86%,内标物α-香附酮的平均绝对回收率为69.4%-74.3%;平均方法回收率醇为59.9%-62.9%,平均方法回收率莪术二酮为64.1%-64.7%,均符合有关要求。通过各组织中莪术醇、莪术二酮含量分析,各组织中均含有两种指标性成分。结论：脾中指标性成分含量较高,心脏中指标性成分含量较低。

Objective：To research tissue distribution of Zedoary oil in rats.Methods：Poured the drug into the stomach of rats,α-Cyperone was chosen as the internal standard,the Plasma drug concentration of Curcumol and Curdione were determined by GC.Results：A good linearity was obtained when the concentration of Curcumol was range from 0.001028 to 0.65780 mg·mL^-1,the concentration of Germacrone was range from 0.002628 to 0.2102mg·mL-1 and the concentration of Curdione was range from 0.0004552 to 0.3642 mg·mL^-1.Precision,Stability and the study of the number of financial freeze were comply with the requirements.The Curcumol＇s absolute recovery of determination methods was 75.8%～79.8%,the Curdione′s was 82.9%～86%.The Absolute recovery of internal standard was 69.4%～74.3%.The Curcumol＇s methods recovery was 59.9%～62.9%,the Curdione＇s was 64.1%～64.7%.Recovery experiments complied with the requirement.There are two component indicators in every organization.Conclusion：There are more component indicators in spleens,and less in hearts.