大孔吸附树脂富集纯化珠子参总皂苷的工艺研究

Enrichment and Purification Process of Total Saponins from Rhizoma Panacis Majoris by Macroreticular Resin

目的:建立大孔吸附树脂富集、纯化珠子参总皂苷的最佳工艺条件。方法:以人参皂苷Re为对照,以总皂苷含量为指标,研究了HPD型大孔树脂吸附和纯化珠子参总皂苷的工艺条件。结果:优化的工艺条件为:样品溶液的浓度为0.13g/mL(生药量/体积);树脂用量为生药量的2倍(质量比);纯化先用水洗至α-萘酚反应呈阴性,改用6BV的60%乙醇洗脱,洗脱速度为2BV/min。结论:HPD-100型非极性大孔树脂对珠子参总皂苷有良好的吸附及纯化性能;本工艺对珠子参总皂苷的纯化收率高且稳定,纯化后总皂苷含量可达60%以上。

Objective：To establish a processing method for enriching and purifying the total saponins from Rhizoma Panacis Majoris by Macroreticular Resin.Methods：With ginseng saponin Re as the standard sample and the total saponins contents as index,to determine the technological Conditions for enriching and purifying the total saponins from Rhizoma Panacis Majoris by HPD Macroreticular Resin.Results：The optimum technical operations were as follows：concentration of the sample was 0.13g/mL（weight of the raw material/volume）;volume of the of the resin was 2 times weight of the raw material;purified with distilled water until the α-Naphthol r.showed negative,and then,with 60% ethanol（6 times the volume of the resin）,and elution speed at 2BV/min.Conclusion：The results showed that HPD-100 non-polarity macroporous resin was suitable for absorbing and purifying the total flavonoid of Rhizoma Panacis Majoris.And purified by HPD-100 macroporous resin,the purity can reach as high as 60%.