实时荧光PCR检测黄疸婴儿尿中人巨细胞病毒感染研究

Detection of HCMV infection in urine of infants by real-time fluorescence PCR

目的探讨实时荧光PCR技术检测尿中人巨细胞病毒(HCMV)在黄疸婴儿HCMV活动性感染的检测价值。方法分别应用实时荧光PCR法和ELISA法检测2585例黄疸婴儿尿HCMV-DNA和血清HCMV-IgM,比较病毒DNA与血清抗体的平行性和敏感性。结果 2585例黄疸婴儿尿中实时荧光PCR检测HCMV-DNA阳性1428例;ELISA检测HCMV-IgM阳性1084例。两种方法阳性符合率为73.88%,实时荧光PCR法和ELISA法阳性检出率分别为55.24%和41.93%,差异有统计学意义(P<0.01);实时荧光PCR法阳性标本的平均拷贝数为7.86×105。结论 HCMV是引起婴儿黄疸的主要原因之一,实时荧光PCR法检测敏感性要高于ELISA法检测,可以有效地提示体内活动性HCMV感染,具有良好的应用价值。

Aim To discuss the value of detection of HCMV in jaundice inants by real-time fluorescenee PCR. Methods Real-time fluorescenee PCR and ELISA were used to detect HCMV-DNA in urine and HCMV-IgM respectively, in serum of 2585 jaundice infants. Results There 1 428 cases positive for HCMV-DNA were detected with a eoineidenee rate of detection was 73.88% and HCMV-IgM was detected in 1084 cases. The psoitive rates of RT-PCR and ELISA were 55.24% and 41.93% ,showing significant difference （P〈0.01） The average copies of RT-PCR was 7.86×10^5. Conclusion HCMV is one of the main pathogen for infant jaundice, the sensitivity of real-time fluorescence PCR is higher than the ELISA.