优势降酚菌的筛选及其生长条件优化研究

Study on Screening of A Dominant Strain for Degradation of Phenol and Optimizing of Its Culture Conditions

采用逐级驯化方法从活化污泥中筛选出可在1800 mg·L^-1苯酚浓度下生长的优势降解菌,命名为JF-6;并通过单因素实验对其生长条件进行了初步研究。结果表明,JF-6可在24 h内将1000 mg.L-1的苯酚完全降解,确定JF-6的最佳生长条件为：温度20-40℃、pH值4-9、NaCl浓度≤4.0%、外加氮源为蛋白胨、装液量40-100 mL·（250mL）^-1。

The phenol-degrading strain named JF-6, which is screened from activation sludge, can grow in inorganic salt medium with 1800 mg ·L ^-1 phenol. The culture conditions are investigated by single factor experiment. The results show that the degradation rate of phenol reaches 100% at phenol concentration of 1000 mg. L 1. The optimum culture conditions are obtained as follows： temperature 20-40℃,pH value 4-9, NaCl concentration≤4.0%, DO （broth content） 40-100 mL of 250 mL shake flask,with peptone as additional nitrogen source.