酶解法定量测定葡聚糖的研究

Study on Quantitative Determination of Dextran by Enzymatic Hydrolysis

以葡聚糖标准溶液为底物,用DNS法测定其经萄聚糖酶酶解产生的还原糖的量来定量推算葡聚糖的含量。结果表明,葡聚糖酶对葡聚糖的分解率为56.0%;回收率为(100±5)%;与Roberts-铜法及Haze-酒精沉淀法比较,测定葡聚糖含量误差均不超过±10%。为葡聚糖定量测定提供了操作简便、重复性好、污染小的测定方法。

With dextran standard solution as the substrate,the quantity of reducing sugar obtained through its hydrolysis by dextranase was determined by DNS spectrophotometry to calculate the content of dextran quantiatively. The results showed that the decomposition rate of dextran by dextranase was 56.0 %, and the recovery rate of dextran was （100±5）%, determination deviation of dextran content was no more than ± 10 % compared with those of Roberts-copper method and Haze-alcohol precipitation method. This study provided a rapid and easy quantitative determination method for dextran.