摘要
以从野生五唇兰植株上分离筛选到的非致病真菌为研究对象,根据形态特征进行种类鉴定,用羧甲基纤维素钠培养基和无机磷培养基进行培养,筛选出具有解纤维和解磷能力菌株,再将菌株接种到五唇兰组培苗上。结果表明:在14株五唇兰非致病真菌中,同时具有解纤维和解磷能力的菌株共10个;除R06和R09菌株外,其余菌株对五唇兰组培苗生长均有促进作用,其中RH18、R03和R14菌株处理的五唇兰组培苗鲜重增长率极显著高于未接菌对照,RH19、R02和S02菌株处理的五唇兰组培苗鲜重增长率显著高于未接菌对照,R05和R002菌株处理与未接菌对照无显著差异。
Doritis pulcherrima＇s no-pathogens were studied and the species identifications were given by morphological character. We screened out fiber-dissolving and phosphate-dissolving fungus by CMC-Na medium and Ca3 （PO4）2 medium in no-pathogens isolated from D. pulcherrima Lindl. Phosphate-dissolving and fiber-dissolving fungus were inoculated on seedling in vitro. The results showed that 10 strain no-pathogens in 14 had the abilities of fiber-dissolving and phosphate-dissolving;the promoting effect of phosphate-dissolving and fiber-dissolving fungi higher than control, excepted R06 and R09;fresh growth rate of D. pulcherrirna seedlings inoculated RH18; R03, R14 were marked significant than control;inoculated RH19, R02, S02 were significant than control. The difference between R05, R002 and control were not significant.
出处
《北方园艺》
CAS
北大核心
2010年第7期81-85,共5页
Northern Horticulture
基金
国家自然科学基金资助项目(30860233)
中央级公益性科研院所基本科研业务费专项资金资助项目(ZS055)
海南省研究生创新基金资助项目(Hxwsy2008-07)
海南大学科技基金资助项目(Rnd0612)
关键词
五唇兰
解纤维能力
解磷能力
菌根真菌
Doritis pulcherrirna Lindl.
fiber- dissolving ability
phosphate-dissolving ability
myorrhizal fungus
