摘要
以海藻酸钠、明胶为载体,戊二醛为交联剂,对香菇纤维素酶进行固定化。研究了海藻酸钠浓度、明胶浓度、氯化钙浓度、给酶量、戊二醛浓度及交联时间对固定化酶的影响,并对固定化酶的最适反应温度、最适pH、温度稳定性等酶学性质进行了测定。结果表明:海藻酸钠和明胶的最佳浓度分别为3.5%和3.0%,戊二醛浓度为1.0%。与游离酶相比,固定化酶最适反应pH向酸性方向移动了0.4,最适反应温度提高了5℃,并且固定化酶具有良好的贮存稳定性。
Cellulase of Lentinula edodes was immobilized on sodium alginate-gelatin carried with glutaraldehyde. The impact of the concentration of sodium alginate, gelatin concentration, amount of calcium chloride concentration, amount of the cellulase, amount of glutaraldehyde concentration and cross linking time on immobilization were analyzed. The thermostability,the optimum temperature and pH value were studied. The results showed that the optimal concentration of sodium alginate and gelatin were 3. 5% and 3. 0% respectively and the concentration of glutaraldehyde was 1.0%. In comparision with the free enzyme, the optimal pH of the immobilized cellulase shifted 0.4 to acidity and the optimum reaction temperature had increased by 5℃. The storage stability of the immobilized enzyme was obviously increased compared to that of the free one. Key words：cellulase;sodium alginate;gelatin;immobilizatiomstability
出处
《北方园艺》
CAS
北大核心
2010年第7期126-129,共4页
Northern Horticulture
基金
山东大学威海分校科研资助项目(A09017)
关键词
海藻酸钠
明胶
纤维素酶
固定化
稳定性
cellulase
sodium alginate
gelatin
immobilization
stability
