摘要
对太行山东麓油松种子园内40个无性系之间的遗传多样性进行分析,并用UPGMA聚类分析就无性系间亲缘关系进行研究。结果表明:从150个10 mer随机引物中筛选出11个,共检测到95个位点,多态位点有77个,多态位点率为81.05%;Nei s遗传多样性指数和Shannon信息指数平均值分别为0.3881和0.5678,各引物的多样性指数差别较大,遗传变异在基因组中分布不均衡;聚类分析结果证明无性系间的遗传亲缘关系较近,遗传背景狭窄。
The thesis used RAPD molecule marker to estimate the genetic diversity of seed orchard of Pinus tabulaeformis. The results showed that 11 random primers produced 95 loci in total, 77 of which were polymorphic loci,the percentage was 81.05%. The genetic background eomplexity and diversity of seed orchard of Pinus tabulaeformis were shown clearly. Nei＇s genetic diversity analysis showed the efficiency number of alleles was 0. 3881 and Shannoris information index was 0. 5678 on average. Largely contrast were in Nei＇s genetic diversity and Shannoris information index, clustering analyses result indicated the genetic relationship was comparatively close and the genetic background was narrow, Genetic variation displayed the special disproportion in genorne
出处
《北方园艺》
CAS
北大核心
2010年第7期130-132,共3页
Northern Horticulture
基金
国家杰出青年基金资助项目(30325006)
邯郸学院硕博启动基金资助项目(2007002)
关键词
油松
种子园
RAPD
遗传多样性
Pinus tabulaefonnis
seed orchard
RAPD markers
genetic diversity
