摘要
在马铃薯茎尖组织培养过程中,对外植体不同的灭菌方法进行研究。结果表明:用流水冲洗24 h,可以降低外植体的污染率和褐化率,同时对材料的生长无较大影响。升汞的灭菌效果虽然好于次氯酸钠,但接种后的效果较差。化学药剂的处理时间为5 min时,虽然污染率稍高,但其接种后效果相对较好。
Effective asepsis material obtained among tissue culture was a basic guarantee for successful tissue culture in plant. As a result, flushing for 24 hours could reduce the polluted rate and brown rate of plant, meantime it was of a sort to growth of plant. The sterilization effect of HgCl2 was better than NaClO but the result of inoculability was worse. Although the polluted rate was high, the effect of inoculability was better when the time of chemical medicine treatment was 5 min.
出处
《北方园艺》
CAS
北大核心
2010年第7期143-144,共2页
Northern Horticulture
基金
马铃薯耐盐变异株系的离体筛选及鉴定资助项目(TDZKSS08003)
关键词
化学试剂
灭菌
时间
two-step
bactericidal
application
