脑脉通有效部位中葛根异黄酮类成分的含量测定

Determination of pueraria isoflavones content in effective parts of Naomaitong

目的:建立脑脉通有效部位中葛根异黄酮类成分的含量测定方法。方法:采用高效液相色谱法,流动相为甲醇-水-冰醋酸,梯度洗脱,流速为1.0 mL/min,检测波长为250 nm。结果:葛根素的平均加样回收率为100.89%,RSD=2.10%,芹糖基葛根素的平均加样回收率为100.23%,RSD=1.06%,3′-甲氧基葛根素的平均加样回收率为101.04%,RSD=1.92%,大豆苷的平均加样回收率为99.82%,RSD=2.02%,大豆苷元的平均加样回收率为102.06%,RSD=1.34%。结论:该方法操作简便、易行,可用于脑脉通中葛根异黄酮的含量测定。

AIM： To establish an HPLC method for determing content of puerarin isoflavones in effective parts of Naomaitong Granules.METHODS： Gradient elution with methanol-water-glacial acetic acid was used as the mobile phase.The flow rate was 1.0 mL/min,and the detection wavelength was set at 250 nm.RESULTS： The average recoveries of puerarin,daizein-8-capiosy（1→6） glucoside,3-methoxyl-puerarin,daidzin,daidzein were 100.89%（RSD = 2.1%）,100.23%（RSD = 1.06%）,101.04%（RSD = 1.92%）,99.82%（RSD = 2.02%）,102.06%（RSD = 1.34%） respectively.CONCLUSION： The method is simple,accurate and can be used for quality control of the effective parts in Naomaitong Granules.