橡胶树丛枝病病原的抗血清制备与应用

Preparation and Application of Antiserium of Causative Agent of Witches' Broom of Hevea Brasiliensis

以感染橡胶树丛枝病病原的长春花为材料，制备得到橡胶丛枝病病原物抽提液，以抽提液为抗原免疫家兔，制备抗血清，经微量沉淀测定，抗血清效价为1：2048。应用橡胶丛枝病抗血清检测橡胶褐皮病，无症苗木检出率达30％～37％；可疑的褐皮病树检出率达85．7％。

Witches'broom of rubber trees (Hevea brasiliensis) is an infectious disease causedby both phytoplasma and bacteria -1ike organism. Tharanthu8 Toseus L. infected with causative agent of witches 'broom of rubber trees was used as materia1 in this study. The freshtender stem barks and 1eaf veins of the infected Tharanthus Toseu were extracted withsolution of 0. 1 mol/1 MgCl2 containing 0. 3 mol/1 glycine,pH 7. 6,and then treated withsnail enzyme and centrifuged differentia11y to prepare extract of causative agent ofWitches'broom of rubber trees. This extract was used as antigen to immunize rabbit forpreparing antiserium. Micro -precipitation test showed that the prepared antiserium had atitre of 1: 2048- Such antiserium was employed to detect brown bast of rubber trees bymeans of micro - precipitation and ELISA indirect method,giving a 30 %-37 % succiss indetecting brown bast of rubber trees among the plants showing no syndromes, and a85. 7 % success among the plants of possible infection