三种黎药的急性毒性研究

Study on the acute toxicity of the aqueous extract of Polygonum hydropiper,Ficus pumila and Evodia lepta

目的:观察辣蓼、薜荔和三叉苦等三种黎药水提物的急性毒性反应。方法:三种黎药水提物对小鼠进行灌胃给药,观察并记录小鼠急性毒性反应和死亡率,采用改良寇氏法测半数致死量LD50。结果:测得辣蓼LD50为128.3g.kg-1(相当于成人一次用量的256.6倍),LD5095%可信限为(111.1～139.7)g.kg-1,10d后存活小鼠体重平均增加22.2%。以最大给药量灌胃薜荔(213.6g.kg-1)和三叉苦(168.0g.kg-1),小鼠无死亡,12d后平均体重分别增加28.3%、28.5%。结论:薜荔和三叉苦口服无毒,而辣蓼毒性很小。

Objective：To investigate the acute toxic reaction of aqueous extract of three Li herbs including Polygonum hydropiper,Ficus pumila and Evodia lepta in mice.Methods：Mice were treated with aqueous extract of three Li herbs including Polygonum hydropiper,Ficus pumila and Evodia lepta by intragastric gavage.Related toxic reaction and mortality of the treated mice were observed,the median lethal dose LD50 were calculated by modified Karber＇s method.Results：The LD50 of aqueous extract of Polygonum hydropiper was 128.3g/kg（equal to 256.6 times of adult＇s administration dosage）,the 95% confidence limit is 111.1-139.7 g/kg,10 days after the administration,the survived mice gained an average weight of 22.2%.Mice that were treated with the maximum administration dosage of Ficus pumila（213.6 g/kg）and Evodia lepta（168.0 g/kg）by intragastric gavage all survived and have gained average weight of 28.3% and 28.5% respectively 12 days later.Conclusion：Ficus pumila and Evodia lepta are non-toxic;while Polygonum hydropiper shows mild toxicity.