乌司他丁对心肺复苏大鼠心功能及心肌组织CD11b、ICAM-1的影响

Effect of UTI on heart functions,CD11b/ICAM-1 in myocardial tissue of CPR rats

目的:观察心肺复苏后大鼠心功能和心肌组织黏附因子CD11b、细胞间黏附分子-1(ICAM-1)水平及心肌细胞超微结构的变化,以及乌司他丁对其的影响。方法:利用窒息法制造心脏骤停大鼠模型,将Sprague Dawley大鼠随机分为对照组、复苏组及乌司他丁组,每组9只。于自主循环恢复(ROSC)2h,测定左室收缩压(LVSP)、左心室舒张末期压(LVEDP)、左心室压力上升、下降最大速率(dp/dt max),采用透射电镜(TEM)观察心肌细胞超微结构变化,ELISA法测定心肌组织CD11b、ICAM-1水平。结果:与复苏组比较,乌司他丁组心功能有所改善,心肌组织病变减轻,CD11b、ICAM-1水平显著下降。结论:乌司他丁可抑制黏附因子CD11b和ICAM-1表达,改善心肺复苏后大鼠心功能,减轻心肌细胞损伤。

Objective：To observe a few indicators of rats after cardiopulmonary resuscitation（CPR）,including the changes in heart function,CD11b and intercellular adhesive molecule-1（ICAM-1）levels of myocardial tissue,ultra-structural changes of cells,as well as the impact of urlinastatin（UTI）on these indicators.Methods：Sprague Dawley rats were randomly divided into three groups：control group,resuscitation group and UTI group,with 9 rats in each group.Cardiac arrest was induced by performing asphyxiation to groups except A and CPR was performed then.Two hours after recovery,tested left ventricle systolic pressure（LVSP）,left ventricle end-diastolic pressure（LVEDP）,maximum rate of increase and decrease in left ventricular pressure（±dp/dt）.The level of CD11b,ICAM-1 in myocardial tissue was determined by the ELISA method,The ultrastntctural damages were observed with transmission electron mictnscope（TEM）.Results：Compared with resuscitation group,heart function in UTI group were improved.The damage of myocardial ultrastructure was ameliorated markedly.The levels of CD11b and ICAM-1 in the UTI group were significantly lower than those in the resuscitation group.Conclusion：UTI can improve myocardial function,inhibits the exhibition of CD11b and ICAM-1,and alleviate the myocardial injury.