摘要
目的；研究成骨细胞与生物活性陶瓷间的关系，方法：选用新西兰大白兔，取胫骨外骨膜组织，经胰蛋白酶及胶原酶分步消化，分离出成骨细胞，用ＰＲＭＩ－１６４０培养液传代培养１３代，通过对培养细胞碱性磷酸酶活性，体外矿化能力测定以及细胞超微结构观察，证实其为典型成骨细胞，将培养分别三种生物活性陶瓷，包括生物活性玻璃陶瓷（ＢＧＣ），羟基磷灰石（ＨＡ）和双相羟基磷灰石（ＨＡ／ＴＣＰ）中培养４８小时后。
Objective: To study the behavior of osteoblast cultured with bioactive materials. Methods:The osteoblasts were isolated from the periosteum of Newzeland Rabbit's tibia, and cultured in RPMI-1640 medium. After 13 subcultures, the cells were identified as osteoblast in vitro by electron microscope, AKP activity and detection of mineral deposition ability.The osteoblasts were subcultured with three bioactive materials: bioactive glass ceramics(BGC), hydroxyapatite(HA), and double phase hydroxyapatite(HA/TCP). After incubation for 48 hours, scan electron microscope,~3H-TdR,XRD,RS and EDXA were performed.Results:The osteoblasts grew on the HA/TCP had a higher proliferation rate and better osteoblastoid shape than those grew on BCG and HA.Conclusion: The degradation of TCP can faciliate growing and proliferating of osteoblasts. It is also beneficial to tissue reconstruction.
基金
美国中华医学基金会(CMB)部分资助
