中线T细胞淋巴瘤的TCR-γ基因重排研究

Detection of T-Receptor γ Chain Gene Rearrangement in Midline T-Cell Lymphomas by PCR Method

目的:对中线T细胞淋巴瘤的克隆性进行研究。方法:用一组针对T细胞受体γ链基因V区片段的家族特异性引物和PCR方法,对11例(22个标本)中线T细胞淋巴瘤病例进行了T细胞受体丁基因重排的检测。结果:22个标本中21个有T细胞受体γ链基因的克隆性重排(94.45％)。在9例原发灶的连续活检和1例原发灶及其转移灶的标本中均未发现增生的克隆家族的改变。结论:中线T细胞淋巴瘤的病变组织中存在T细胞的单克隆性增生,为该肿瘤的T细胞起源提供了分子生物学的证据。在中线T细胞淋巴瘤的疾病过程中(包括转移灶)增生T细胞的家族未发生变化,支持肿瘤的单克隆起源学说。

Objective: To investigate the clonality of midline T-cell lymphoma.Methods: Using polymerase chain reaction method and a panel of family specific primers for the variable regions of TCR- γgene, and totally 11 cases(22 samples) of midline T-cell lymphoma(idline malignant reticulosis) were studied.Results: Family specific monoclonal TCR-γgene rearrangements were found in 21 out of 22 samples(94.45％). There was no change of clonality during the course of disease. Conclusion: There is monoclonal T-cell proliferation in midline T-cell lymphoma and the clonality is not transformed with the advance of the disease. This finding provides an evidence to support the hypothesis of monoclonal origin of neoplasm.