Fascin及VEGF在膀胱移行细胞癌中的表达及意义

Expression and Clinical Significance of Fascin Protein and VEGF in Bladder Transitional Cell Carcinoma

目的:探讨球形肌动蛋白交联蛋白Fascin与血管内皮生长因子(VEGF)在膀胱移行细胞癌(BTCC)组织的表达及与BTCC生物学行为的相关性。方法:采用免疫组织化学SABC法分别检测56例BTCC组织及10例正常膀胱黏膜组织Fascin、VEGF的阳性表达情况。结果:Fascin蛋白及VEGF在10例正常膀胱组织中均不表达,而在56例BTCC组织中阳性表达率分别为73.21%和60.71%,2种组织间Fascin、VEGF表达比较差异均有统计学意义(均P<0.01);随着膀胱癌病理分级和临床分期的增高,Fascin及VEGF表达均增高。Fascin及VEGF阳性表达率在复发患者均高于初发患者,差异有统计学意义(均P<0.01)。在BTCC组织中Fascin与VEGF表达呈正相关(r=0.4769,P<0.01)。结论:Fascin可作为判断BTCC生物学行为的新参考指标,Fascin及VEGF协同促进BTCC的浸润及转移,两者为临床开展肿瘤防治新途径提供新的理论依据。

Objective： To investigate the relationship between the expression of Fascin protein and vascular endothelial growth factor（VEGF）and the biological behavior of bladder transitional cell carcinoma （BTCC）. Methods： The expressions of Fascin and VEGF were examined by SABC （StreptAvidin-Biotin Complex）immunohistochemistry in 56 paraffin-embedded tissue specimens and 10 control samples of normal bladder tissues. Results： The positive expression rates of Fascin and VEGF were 0 in normal bladder tissue. The positive expression rates of Fascin and VEGF were 73.21% and 60.71% in BTCC （P 0.01）. The higher expressions of Fascin and VEGF were related to the tumor grade, clinical stage and recurrence （P 0.01）. The ex- pression of Fascin was closely correlated with that of VEGF in BTCC （r = 0.476 9, P 0.01）. Conclusion： The expression of Fascin may be one of parameters for understanding the biological behavior of BTCC. Fascin protein and VEGF may enhance the influence of the development of BTCC together, which may also provide theoretical foundation of chemopreventive stategy for bladder cancer in the future.