肝细胞癌患者外周血CD95和OX40L的表达

Expression of CD95 and OX40L in Hepatocellular Carcinoma

目的:研究肝细胞癌(HCC)患者外周血CD95和OX40L mRNA的表达情况,并探讨其临床意义。方法:将研究对象的淋巴细胞与相应荧光标记的单克隆抗体反应,经洗涤、固定后,应用双色流式细胞术分析T淋巴细胞表面CD95+CD3+的表达量。采用荧光定量逆转录聚合酶链反应(FQ-RT-PCR)方法检测外周血单个核细胞共刺激分子OX40L mRNA的表达水平。结果:HCC患者外周血CD95+CD3+的表达水平明显高于健康对照组,差异有统计学意义([34±20)%vs(20±7)%,t=2.960,P<0.01];HCC患者外周血单个核细胞OX40L mRNA表达水平明显低于健康对照组,差异有统计学意义(0.46±0.36vs0.86±0.70,t=2.302,P<0.05)。结论:HCC患者外周血CD95及OX40L分子的异常表达在HCC的发生中起重要作用。

Objective： To assess the expression levels of CD95 and OX40 ligand （OX40L） messenger RNA （mRNA） in hepatocellular carcinoma （HCC）, and their clinical values thereof. Methods： The lymphocytes of research objects were mixed with corresponding fluorescence labeled monoclonal antibody （McAb）; CD95 expression by CD3 positive T cells was quantitatively measured by dual color flow cytometry. The expression of OX40L mRNA was detected in peripheral blood mononuclear cells by fluorescence quantitative reverse transcription polymerase chain reaction（FQ-RT-PCR）. Results： The CD95 expression by CD3 positive T cells was significantly higher in patients with HCC （34±20）% compared with that of normal controls （20±7）%（t = 2.960,P 0.01）. The expression level of OX40L mRNA was significantly decreased in patients with HCC compared with that of normal controls （t = 2.302,P 0.05）. Conclusion： These results suggest that abnormal expressions of CD95 and OX40L play crucial roles in peripheral blood of HCC patients at the stage of human hepatocarcinogenesis.