转化生长因子-β亚型在溃疡性结肠炎患者结肠黏膜中的表达

Expression of Transforming Growth Factor-β Isoforms in Colonic Mucosa of Patients with Ulcerative Colitis

目的:探讨转化生长因子-β(TGF-β)亚型在溃疡性结肠炎(UC)患者结肠黏膜中的表达及其在UC发病中的作用。方法:免疫组化Envision法检测UC、肠易激综合征(IBS)患者结肠黏膜中TGF-β亚型的表达,分析其与UC严重程度和病变范围的关系。结果:TGF-β1、TGF-β2的表达在活动期UC高于缓解期UC(Z分别为7.924,7.122,P<0.01)和IBS组(Z分别为36.954,33.485,P<0.01),缓解期UC高于IBS组(Z分别为12.211和10.621,P<0.01);TGF-β3的表达在活动期UC、缓解期UC和IBS组间差异无统计学意义(P>0.05)。在活动期UC中,TGF-β1、TGF-β2的表达与UC的严重程度均呈正相关(rs分别为0.566,0.564,P<0.05),TGF-β3的表达与UC的严重程度无线性相关性(P>0.05);TGF-β1、TGF-β2、TGF-β3的表达与UC病变范围均无线性相关性(P>0.05);疾病严重程度和病变范围呈正相关(rs=0.337,P<0.05)。结论:在UC结肠黏膜中TGF-β1、TGF-β2表达增强,与UC的严重程度密切相关。TGF-β1、TGF-β2可作为反映UC疾病活动度的指标。

Objective： To investigate the expression of transforming growth factor-β （TGF-β） isoforms in colonic mucosa of ulcerative colitis（UC）, and its role in the incidence of UC thereof. Methods： The expression of TGF-β isoforms was detected in colonic mucosa by immunohistochemical Envision method in UC patients, and the patients with irritable bowel syndrome （IBS） were used as controls. The relationship was analyzed between the expression of TGF-β isoforms and the degree of severity and extent of the disease. Results： There were statistical differences in the expression of TGF-β1 and TGF-β2 between the active stage UC, remission stage UC and IBS groups（P 0.01）. The expression was significantly higher in active stage UC group than that in remission stage UC and IBS groups（P 0.01）. The expression was significantly higher in remission stage UC group than that in IBS group（P 0.01）. However, there was no statistical difference in the expression of TGF-β3 between active stage UC, remission stage UC and IBS groups （P 0.05）. The expression of TGF-β1 and TGF-β2 had positive correlation with the degree of severity（P 0.05）. However, the expression of TGF-β3 had no linear correlation with the degree of severity（P 0.05）. There was no linear correlation on the expressions of TGF-β1, TGF-β2 and TGF-β3 with extent of the disease （P 0.05）. The degree of severity had positive correlation with extent of the disease （P 0.05）. Conclusion： The expressions of TGF-β1 and TGF-β2 were enhanced in colonic mucosa of UC, and were correlated with the pathogenesis of UC. The expressions of TGF- β1 and TGF-β2 may serve as markers for assessing disease activity of UC.