白细胞介素-6对肝细胞载脂蛋白M表达的影响

Interleukin-6 Inhibits the Expression of Apolipoprotein M in HepG2 Cells

目的:研究白细胞介素(IL)-6对人肝母细胞瘤细胞(HepG2)载脂蛋白(apo)M表达的影响及两者在糖尿病病程中的作用。方法:体外培养HepG2细胞,分别以不同浓度的IL-6干预细胞24h,提取细胞总RNA,应用逆转录聚合酶链反应(PCR),琼脂糖凝胶电泳和实时荧光定量PCR检测apoM的表达,并同时检测另一种载脂蛋白apoA-I的表达。用同样的干预方式,检测IL-1α对apoM表达的影响。结果:IL-6(1.25、2.5、5、10、20、40、80μg/L)对HepG2细胞apoM的表达具有抑制作用,与对照组(0μg/L)比较差异有统计学意义(F=10.778,P<0.01),但是IL-6的干预对apoA-I表达水平的影响差异无统计学意义(F=2.004,P>0.05),IL-1α(1.25、2.5、5、10、20、40、80μg/L)对apoM表达的影响差异亦无统计学意义(F=2.038,P>0.05)。结论:IL-6能够抑制HepG2细胞apoM的表达,这可能是2型糖尿病患者脂代谢异常以及大血管并发症的发病机制之一。

Objective： To investigate the effect of interleukin-6 （IL-6） on apolipoprotein （apo） M expression in a human hepatoblastoma cell line （HepG2）. Methods： HepG2 cells were cultured and incubated with different concentrations of IL-6 （0, 1.25, 2.5, 5, 10, 20, 40 and 80 μg/L） for 24 hours. After the incubations, total RNAs were extracted and applied to reverse transcript PCR and real-time quantitative PCR to detect the expression levels of apoM and apoA-I. The effect of IL-1α on apoM expression was also determined. Results： IL-6 significantly inhibited the expression of apoM （F = 10.778, P 0.01）. Whereas IL-6 did not influence the expression of apoA-I（F=2.004, P 0.05）. IL-1α（0, 1.25, 2.5, 5, 10, 20, 40 and 80 μg/L） did not decrease the expression of apoM （F = 2.038, P 0.05）. Conclusion： IL-6 inhibits apoM mRNA transcription, which may contribute to the pathogenesis of abnormal lipid metabolism and macrovascular complications in type 2 diabetes.