HBx和VEGF与HBV相关性肝癌组织血管生成及转移的关系

The correlation of HBx and VEGF expression with angiogenesisand metastasis in HBV related hepatocelular carcinoma

目的探讨乙型肝炎病毒X蛋白(HBx)和血管内皮生长因子(VEGF)与HBV相关性肝癌组织血管生成及转移的关系。方法选择84例HBV相关性肝癌组织和22例非HBV相关性肝癌组织,免疫组织化学法检测HBx、VEGF及血管内皮细胞表面抗原(CD34)表达,光镜下记录微血管计数(MVD)。结果84例HBV相关性肝癌组织中HBx阳性表达率为73.81%(62/84),阴性率为26.19%(22/84);62例HBx阳性表达组中的VEGF阳性率为74.19%(46/62),明显高于22例HBx阴性组36.36%(8/22)和非HBV相关性肝癌组36.36%(8/22)(P<0.05);HBx阴性表达的HBV相关性肝癌组和非HBV相关性肝癌组中VEGF阳性表达差异无统计学意义(P>0.05);HBx和VEGF的表达呈正相关(r=0.552,P<0.01);二者在高分化肿瘤组织中的表达高于低分化组织(P<0.05),且转移组表达高于无转移组;HBx阳性表达组平均MVD值明显高于HBx阴性组和非HBV相关性肝癌组(P<0.01),有转移组MVD高于无转移组(P<0.01);有门脉侵犯组高于非侵犯组(P<0.05)。结论HBx和VEGF广泛表达于HBV相关性肝癌组织中,二者呈正相关;HBx可能通过上调VEGF的表达在HBV相关性肝癌组织血管生成及转移中起促进作用。

Objective To investigate the corrrelation between HBx,VEGF and microvessel density（MVD）expression in HBV related hepatocelular carcinoma angiogenesis and metastasis.Methods Immunohistochemistry was used to detect the expression of HBx,VEGF and CD34 in 84 HBV related hepatic carcinoma and 22 non-HBV related hepatic carcinoma tissues.Results The positive rate of HBx in HBV related hepatocellular carcinoma tissues was 73.81%（62/84）.Among these,74.19% HBx positive samples also showed VEFG positive,which was significantly higher than that in 22 HBx negative expression hepatic carcinoma tissues（36.36%,8/22） and in 22 non-HBV related hepatic carcinoma tissues（36.36%,8/22）,These was no statistical significance of VEGF expression in HBx negative expression and non-HBV related hepatic carcinoma tissues.The positive correlation（r=0.552,P0.01）.The expression of HBx and VEGF were upregulated in well histodifferentiated cancer tissues（P0.05） and metastasis.MVD expression also inreased in HBx positive tissues comparing with the HBx negative expression and non-HBV related hepatic carcinoma tissues（P0.01）.MVD expression in cases showhg metastasis and portal vein invasion was significantly activated（P0.01）.Conclusion HBx and VEGF specifically expressed in HBV related hepatocellular carcinoma tissues which suggested the HBx may play an important role in hepatocelular carcinoma angiogenesis by upregulating VEGF.