慢性脊髓压迫减压后的缺血再灌注损伤

Ischemia-reperfusion injury after decompression in chronic spinal cord compression

目的探讨缺血再灌注损伤是否为慢性脊髓压迫症减压后不明脊髓功能丧失的致伤因素。方法成年新西兰白兔96只,随机分为A组(假手术组),B组(缺血再灌注组),C组(慢性压迫减压组)。于0h、0.5h、6h、12h、24h,48h各时间点每组分别取4只动物,检测MDA、CAT、SOD、GSH-Px表达水平;每组于0h及48h时各取4只动物取材行凋亡细胞计数。结果B组MDA、CAT、SOD、GSH-Px于0.5h、6h、12h、24h表达水平明显高于A组;C组MDA、CAT、SOD、GSH-Px于各时间点表达水平均明显高于A组,且C组组间各时间点表达水平无明显区别;C组0h及48h时TUNEL阳性细胞数量无明显差异。结论慢性脊髓压迫减压后减压局部并无明显缺血再灌注损伤标志,缺血再灌注并非减压后脊髓功能丧失的原因。

Objective To investigate if ischemia-reperfusion（I/R） injury is the correlated injury factor to unknown deprivation of spinal cord function after decompression in chronic spinal cord compression.Methods All the 96 New Zealand white rabbits were randomized as follows：Group A（negative control group）,Group B（I/R group）,Group C（decompression group）.MDA、SOD、CAT、GDH-Px were detected on 4 rabbits in each group at 0 h,0.5 h,6 h,12 h,24 h,48 h.Apoptosis cell numbers were analysis on 4 rabbits in each group at 0 h and 48 h.Results MDA,CAT,SOD,GSH-Px levels in Group B were much higher than those in Group A at 0.5 h,6 h,12 h,24 h,and the levels in Group C were much higher than those in Group A at each time point,and there were no differences among each time point in Group C.TUNEL stain showed the apoptosis cell number had no significant difference between 0 h and 48 h in Group C.Conclusion There is no significant I/R injury evidence locally after decompression in chronic spinal cord compression,and I/R injury is not the cause of deprivation of spinal cord function after spinal cord decompression.