中药单体化合物光甘草定的体外抗菌活性研究

Antimycobacterial and antibacterial activity of glabridin from Glycyrrhiza uralensis Flsch in vitro

选取传统中药甘草提取单体化合物光甘草定,通过MABA药物敏感性实验方法研究其对结核分枝杆菌H37Rv(ATCC27294)和H37Ra(ATCC25177)的体外抗菌活性,并通过NCCLS标准方法测定了光甘草定对金黄色葡萄球菌ATCC25923和ATCC29213、粪肠球菌ATCC29212和大肠杆菌ATCC25922的最低抑菌浓度(MIC)。结果表明:单体化合物光甘草定对结核分枝杆菌H37Rv(ATCC27294)和H37Ra(ATCC25177)有很好的抗菌活性,最低抑菌浓度均为25μg/m;光甘草定对实验中检测的金黄色葡萄球菌、粪肠球菌和大肠杆菌也有抗菌活性,并且对革兰氏阳性杆菌的抗菌活性高于革兰氏阴性杆菌。本研究为光甘草定的进一步开发利用和抗菌机制研究打下了基础。

Glabridin is an active flavonoid constituent from Glycyrrhiza uralensis Flsch,a traditional Chinese medicine. The antimycobacterial susceptibility of glabridin was determined by a microdilution alamar blue Assay （MABA） against Mycobacterium tuberculosis H37Rv （ATCC 27294） and H37Ra （ATCC 25177）,and the antibacterial activities were determined by broth microdilution against Staphylococcus aureus ATCC25923 and ATCC29213,Enterococcus faecalis ATCC29212,and Escherichia coli ATCC25922. The results showed that glabridin had a minimal inhibitory concentration （MIC） of 25 μg/mL against M. tuberculosis H37Rv and H37Ra,respectively. Glabridin was active against S.aureus,E.faecalis,and E.coli,and was more effective against Gram-positive bacteria than against Gram-negtive bacteria. These results will pave the way to further development and exploring the mechanism of glabridin against bacteria.