天麻素对乙醇诱导肝细胞株L02细胞损伤的影响

The effect of gastrodine on hepatitic cell line L02 injury induced by ethanol

目的:探讨天麻素对乙醇诱导肝细胞株L02损伤的影响。方法:将人肝细胞株L02培养,加入不同浓度的乙醇,采用MTT比色法确定乙醇作用的浓度。用流式细胞仪测定细胞活性氧簇(ROS)及线粒体膜电位的改变;采用反相高效液相色谱(RP-HPLC)分析法检测细胞ATP含量。结果:乙醇(25 mL/L)作用36 h可损伤肝细胞,细胞内ROS的水平明显增加;而细胞线粒体膜电位及肝细胞中ATP的含量则显著降低(P<0.01)。天麻素具有减轻细胞损伤的作用,并可降低损伤的肝细胞内ROS的含量,增加细胞线粒体膜电位和ATP的水平。结论:天麻素可抑制乙醇诱导的肝细胞损伤及脂质过氧化反应,改善线粒体功能,增加能量合成,发挥保护肝细胞的作用。

AIM：To explore the the effect of gastrodine on hepatitic cell line L02 injury induced by ethanol.METHODS：The growing L02 cells were treated with different concentrations of alcohol.For screening the proper concentration of alcohol,the proliferation of the cells were measured by MTT colorimetry.The level of reactive oxygen species（ROS） and change of mitochondrial membrane potential in cells were tested by flowcytometry.Reversed-phase high-performance liquid chromatography（RP-HPLC） was applied to assay ATP content in cells.RESULTS：The cells were injuried when treated with 25 mL/L alcohol for 36 h.Compared with the control group,the ROS level in cells markedly increased while mitochondrial membrane potentia and ATP content in cells significanty decreased（P0.01）.Gastrodine obviously inhibited cell damage,reduced ROS level,and increased mitochondrial membrane potential and ATP content in cells.CONCLUSION：Above results suggest that gastrodine may suppress cellular injury and lipid peroxidation induced by ethanol,improve mitochondrial function and raise ATP level,which play an important role in protecting hepatocytes.