期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

哮喘大鼠淋巴液与血液CD4^+CD25^+Treg及IL-10、TGF-β水平比较和地塞米松干预的影响 被引量：9

The comparison of CD4^+CD25^+Treg,IL-10 and TGF-β from lymph and blood in bronchial asthmatic rat and the effect of dexamethasone on it
下载PDF
导出
摘要 目的:观察CD4+CD25+Foxp3+调节性T细胞、IL-10、TGF-β在哮喘大鼠淋巴液与血液中的水平,以及地塞米松干预的影响,探讨其与哮喘发病的关系。方法:建立大鼠哮喘模型,收集激发后0、24、48 h淋巴液、血液中淋巴细胞,采用流式细胞术(FCM)检测淋巴液及血液中CD4+CD25+Foxp3+T细胞百分率;采用ELISA方法检测血浆及淋巴液中IL-10、TGF-β水平。结果:哮喘组淋巴液和血液的CD4+CD25+Foxp3+T细胞百分率水平、血浆IL-10和TGF-β浓度在各时间点均低于对照组和治疗组(P<0.05);哮喘组淋巴液中CD4+CD25+Foxp3+T细胞百分率水平、IL-10浓度在不同时间点均高于血液水平(P<0.05),而TGF-β浓度则低于血液水平(P<0.05);治疗组淋巴液和血液中CD4+CD25+Foxp3+T细胞百分率水平、血浆IL-10和TGF-β浓度与对照组相比无显著性差异(P>0.05)。结论:哮喘大鼠淋巴液中存在CD4+CD25+Foxp3+调节性T细胞数量以及功能失调,且淋巴液中CD4+CD25+Foxp3+调节性T细胞水平显著高于其血液水平,地塞米松可能通过影响CD4+CD25+Foxp3+调节性T细胞数量以及功能而发挥治疗作用。 AIM：To observe the expression of CD4＋CD25＋Foxp3＋Treg,IL-10 and TGF-β in lymph and blood,as well as the effect of dexamethasone on it and investigate the relationship between them.METHODS：Lymph and blood samples of 0 h,24 h,48 h after the last challenge were collected from the rat model of asthma.The percentage of CD4＋CD25＋Foxp3＋Treg were detected by flow cytometer（FCM）,while the levels of IL-10 and TGF-β were determined by ELISA.RESULTS：The percentage of CD4＋CD25＋Foxp3＋Treg in the blood and the levels of IL-10 and TGF-β in the plasma in cases of asthma group were significantly lower than that of the control and therapy group at different time points（P0.05）;The percentage of CD4＋CD25＋Foxp3＋Treg and the level of IL-10 in lymph were significantly higher than that of blood（P0.05）,however,the level of TGF-β in lymph were significantly lower than that of blood（P0.05）;There were no significant differences which the percentage of CD4＋CD25＋Foxp3＋Treg in the blood and the levels of IL-10 and TGF-β in the plasma between control group and therapy group（P0.05）.CONCLUSION：There is a disbalance both quantity and function of CD4＋CD25＋Foxp3＋ in the lymph of bronchial asthmatic rat,and the percentage of CD4＋CD25＋Foxp3＋Treg in lymph are significantly higher than that of blood,which dexamethasone may effectively increase the percentage of CD4＋CD25＋Foxp3＋Treg in bronchial asthmatic rat.
作者 赵志旭 冯学斌 石涛 杨成 张立霞
机构地区 滨州医学院儿科学教研室 滨州医学院实验室中心
出处 《细胞与分子免疫学杂志》 CAS CSCD 北大核心 2010年第3期238-241,共4页 Chinese Journal of Cellular and Molecular Immunology
基金 山东省教育厅科技计划项目(J07YE14)
关键词 哮喘 调节性T细胞 白细胞介素10 转化生长因子Β asthma regulatory T cell interleukin-10 transforming growth factor-β
分类号 R562.2 [医药卫生—呼吸系统]
  • 相关文献

参考文献12

  • 1Stock P,DeKruyff RH,Umetsu DT.Inhibition of the allergic response by regulatory T cells[J].Curr Opin Allergy Clin Immunol,2006,6(1):12-16.
  • 2Umet su DT,Dekruyff RH.Tmmune dysregulation in asthma[J].Curr Opin Immunol,2006,18(6):727-732.
  • 3迟磊,符州,戴继宏,王莉佳.过敏性哮喘大鼠模型的建立[J].重庆医学,2003,32(4):429-431. 被引量：60
  • 4Cavassani KA,Campanelli AP,Moreira AP,et al.Systemic and local characterization of regulatory T cells in chronic fungal infection in humans[J].J Imnnmol,2006,177(9):5811-5818.
  • 5Hartl D,Koller B,Mehlhorn AT,et al.Quantitative and functional impairment of pulmonary CD4~+ CD25~(hi) regulatory T cells in pediatric asthma[J].J Allergy Clin Immunol,2007,119(5):1258-1266.
  • 6Lee JH,Yu HH,Wang LC,et al.The levels of CD4~+ CD~25+ regulatory T cells in paediatric patients with allergic rhinitis and bronchial asthma[J].J Clin Exp Immunol,2007,148(1):53-63.
  • 7Strickland DH,Stumbles PA,Zosky GR,et al.Reversal of airway hyperresponsiveness by induction of airway mucosal CD4~+ CD~25+ regulstory T cells[J].J Exp Med,2006,203(12):2649-2660.
  • 8Mempel TR,Henrickson SE,Von Andrian UH.T-cell priming by dendritic cells in lymph nodes occurs in three distinct phases[J].Nature,2004,427(6970):154-159.
  • 9李营营,冯学斌,吴福玲,邢朝品,杨成.支气管哮喘大鼠淋巴液Th1/Th2类细胞因子分布特点及其与BALF、血浆水平比较[J].中国免疫学杂志,2009,25(10):952-953. 被引量：9
  • 10邢朝品,冯学斌,宋晓冬,张丽霞,刘文波.支气管哮喘大鼠淋巴液中淋巴细胞线粒体跨膜电位变化及地塞米松对其干预的影响[J].滨州医学院学报,2009,32(4):249-252. 被引量：4

二级参考文献17

  • 1刘艳凯.淋巴回流在机体稳态调节中的意义[J].中国微循环,2006,10(4):307-310. 被引量：12
  • 2Bisset LR , Schmid-Grendelmeier P. Chemokines and their receptors in the pathogenesis of allergic asthma : proga'ess and perspective [ J]. Curr Opin Pulm Med, 2005,11 ( 1 ) :35-42.
  • 3Woodside KJ, Hu M, Meng T,et al. Differential effects of Interleukin- 2 blockade on apoptosis in naive and activated human lymphocytes [ J ]. Transplantation,2003, 75 (10) : 1631-1635.
  • 4Verburg J, Hollenbeck PJ. Mitochondrial membrane potential in axons increasesWith local nerve growth factor orsemaphor insigaling [ J]. Neurosci ,2008,28 (33) :8306-8315.
  • 5Pak HN , Qayyum M , Kim DT, et al. Mesenchymal stem cell injection induces cardiac nerve sprouting and increased tenascin expres sion in a Swine model of myocardial infarction [ J ]. Cardiovasc Elec trophysiol, 2003, 14 (8) :841-848.
  • 6L undy SK, Berlin AA, M artens TF, et al. Deficiency of regulatry B ceils increases allergic airway inflammation [ J ]. Inflamm Res, 2005, 54 (12) : 514-521.
  • 7Tong JK, Hozefa S, Bandulwala, et al. Fas-positive T cells regulate the resolution of airway inflammation in a murine model of asthma [J]. J Exp Med,2006, 203(5):1173-1184.
  • 8Ray A, Cohn L. Altering the Th1/Th2 balance as a therapeutic strategy in asthmatic diseases [J]. Curt Opin Investig Drugs,2000; 1(4) :442-448.
  • 9Venkayya R, Lain M, Willkom Met al. The Th2 lymphocyte products IL-4 and IL-13 rapidly induce airway hyper-responsiveness through direct effects on resident airway cells[J]. Am J Respir Cell Mol Biol, 2002 ; 26 (2) :202-208.
  • 10Pala P, Message S D, Johnston S Let al. Increased aeroallergen-specific interleukin4-producting T cells in asthmatic adults[ J ]. Clin Exp Allergy, 2002 ; 32(12) : 1739-1744.

共引文献65

同被引文献65

  • 1薛克营,柯明耀,姜燕,陈玲玲,吴雪梅,谢红旗.吸入激素对哮喘患者外周血CD4^+CD25^+调节性T细胞水平及Foxp3 mRNA表达的影响[J].中国呼吸与危重监护杂志,2011,10(3):285-288. 被引量：3
  • 2王洪瑞,杨敬平.变应性鼻炎、哮喘患者T细胞亚群、白介素水平及肺功能测定[J].临床肺科杂志,2006,11(2):174-175. 被引量：10
  • 3刘雨生,赵淑云,季静娟,翟志敏,童先宏,骆丽华,栾红兵.原因不明复发性流产患者调节性T淋巴细胞比例及功能的测定[J].中华妇产科杂志,2007,42(3):176-179. 被引量：12
  • 4肖承棕.中医妇科临床研究[M].北京:人民卫生出版社,2009:89.
  • 5Lewkowich IP,Herman NS, Schleifer KW, et al. CD4 + CD25 + Tcells protect against experimentally induced asthma and alter pulmonarydendritic cell phenotype and function [ J]. J Exp Med,2005,202(11): 1549 -1561.
  • 6Park SK, Cho MK, Park HK, et al. Macrophage migration inhibitoryfactor homologs of anisakis simplex suppress Th2 response in allergicairway inflammation model via CD4 + CD25 + Foxp3 + T cell recruit-ment[J]. J Immunol, 2009 , 182( 11) : 6907 -6914.
  • 7Chen Y, Shen S, Gorentla BK, et al. Murine regulatory T cellscontain hyperproliferative and death-prone subsets with differentialICOS expression.J]. J Immunol, 2012, 188(4) : 1698 - 1707.
  • 8Presser K, Schwinge D, Wegmann M, et al. Coexpression of TGF-betal and IL-10 enables regulatory T cells to completely suppress air-way hyperreactivity[J]. J Immunol, 2008,181 ( 11 ) ; 7751 -7758.
  • 9Birebent B, Lorho R, Lechartier H, et al. Suppressive properties ofhuman CD4+ CD25 + regulatory T cells are dependent on CTLA-4expression[ J]. Eur J Immunol,2004, 34( 12) : 3485 - 3496.
  • 10Dong C, Juedes AE, Temann UA, et al. ICOS co-stimuiatory receptoris essential for T-cell activation and function [ J ]. Nature, 2001,409(6816) : 97-101.

引证文献9

二级引证文献58

细胞与分子免疫学杂志
2010年 第3期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部