载银纳米抗菌复合骨填充材料体外抗菌性能及生物相容性研究

Experimental research on in vitro antibacterial property and biocompatibility of bone filling materials of TiO_2-Ag-nHA/PA66

[目的]探讨载银纳米抗菌复合骨填充材料其体外抗菌性能及生物相容性。[方法]采用抑菌环试验、菌落数试验检测材料对金黄色葡萄球菌及大肠杆菌的体外抗菌效果,采用扫描电镜观察材料对金黄色葡萄球菌及大肠杆菌的抗粘附效果。并采用MTT法检测细胞毒性及急性溶血实验评价载银纳米抗菌复合骨填充材料的生物相容性。[结果]抑菌环试验显示第1 d时载银纳米抗菌复合骨填充材料对金黄色葡萄球菌和大肠杆菌的抑菌环直径均最大,分别为(23.6±1.14)mm和(18.8±0.84)mm,随后抑菌环直径随时间延长而缩小,抑菌环在金黄色葡萄球菌和大肠杆菌中的持续时间分别为33、24 d;菌落数试验显示细菌与载银纳米抗菌复合骨填充材料接触24 h后,对金黄色葡萄球菌和大肠杆菌的抗菌率分别为94.18%和85.96%;扫描电镜发现实验组材料表面黏附的细菌明显少于对照组。MTT法测定示载银纳米抗菌复合骨填充材料毒性分级为1级,急性溶血实验示载银纳米抗菌复合骨填充材料溶血率为0.28%。[结论]载银纳米抗菌复合骨填充材料有良好的生物相容性,对金黄色葡萄球菌及大肠杆菌有明显抗菌作用,无明显细胞毒性和红细胞破坏性。

[Objective]To investigate the in vitro antibacterial effect and biocompatibility of the bone filling materials of TiO2-Ag-nHA/PA66.[Method]The inhibition ring test and plate-counting method were used to evaluate anti-bacterial performance against staphylococcus aureus ATCC25923 and escherichia coli ATCC25922.The effects of antiadhesion of staphylococcus aureus and escherichia coli were observed by scanning electron microscope（SEM）.The cytotoxicity was detected via MTT and the biocompatibility of biofilm was evaluated by acute haemolysis test.[Result]The inhibition zone diameter of staphylococcus aureus and escherichia coli reaches the maximum at the first day[（23.6±1.14） mm and（18.8±0.84 mm）].The diameter of inhibition zone was lessening with the time going.It could effectively inhibite the survival and growth of staphylococcus aureus and escherichia coli within 33 and 24 days.The bacteria killing rate was 94.18% and 85.96%.Scanning electron microscope showed that the adhered bacteria in the experimental group were obviously fewer than in the control group.The MTT graded the cytotoxicity of the bone filling materials of TiO2-Ag-nHA/PA66 as 1.The acute haemolysis assay showed that the hemolytic rate of bone filling materials of TiO2-Ag-nHA/PA66 was 0.28%.[Conclusion]The bone filling materials of TiO2-Ag-nHA/PA66 has good biocompatibility,significant antibacterial property against staphylococcus aureus and escherichia coli.No obvious cytotoxicity or erythrocyte destruction was found.