丹酚酸B联合高氧液对兔肢体再灌注损伤保护作用

Effect of salvianoli acid B and hyperoxic solution on experimental ischemia reperfusion injury of extremities

[目的]探讨高氧液和丹酚酸B对兔肢体再灌注损伤的保护作用。[方法]选用健康新西兰家兔24只,随机分为4组,在缺血前从耳缘静脉推注等量的生理盐水(A组)、高氧液(B组)、丹酚酸B(C组)或高氧液加丹酚酸B(D组),夹阻股动静脉,建立肢体缺血再灌注损伤模型,在缺血前和再灌注4 h抽血检测丙二醛(MDA)和超氧化物岐化酶(SOD),取腓肠肌作病理检测。[结果]再灌注损伤后血清丙二醛浓度较前明显升高,丹酚酸B组、高氧液组以及丹酚酸B联合高氧液组的MDA升高均受到抑制(P<0.01),以丹酚酸B联合高氧液组最为明显;血清超氧化物岐化酶活性较前明显降低,丹酚酸B组、高氧液组以及丹酚酸B联合高氧液组的SOD降低均受到抑制(P<0.01),以丹酚酸B联合高氧液组最为显著。骨骼肌HE染色见丹酚酸B联合高氧液组骨骼肌损伤程度最轻。[结论]丹酚酸B联合高氧液和对兔肢体缺血再灌注损伤具有一定的保护作用,且两者有协同作用。

[Objective]To investigate the effect of salvianoli acid B and hyperoxic solution on experimental ischemia reperfusion injury of extremities.[Method]Twenty-four healthy New Zealand rabbits were divided into 4 groups randomly.Equivalent 0.9%Nacl（group A）,hyperoxic solution（group B）,salvianoli acid B（group C）,salvianoli acid B and hyperoxic solution（group D） were injected through ear edge vein before ischemia.The femoral artery and vein were blocked and the model of ischemia reperfusion injury of extremities was set up.Before ischemia and after 4 hour＇s reperfusion,the blood samples were taken to measure the levels of blood malondialdehyde（MDA） and superoxide dismutase（SOD）.The gastrocnemius muscles were observed by pathologic test.[Result]Serum enzymatic activity of MDA improved obviously.Salvianoli acid B and hyperoxic solution could prevent the improvement of it（P〈0.01）.Serum enzymatic activity of SOD decreased obviously.Salvianoli acid B and hyperoxic solution could prevent the decreasing of it（P〈0.01）.HE test showed the lightest degree of skeletal muscle injury in group D.[Conclusion]Salvianoli acid B and hyperoxic solution can decrease damage in ischemia reperfusion injury,and they have synergistric effect.