不同时间给予大蒜油对四氯化碳急性肝损伤的保护效果

Protective effect of garlic oil given at different time against acute liver injury induced by

目的 观察并比较不同时间给予大蒜油对四氯化碳（carbon tetrachloride,CCl4）引起的急性化学性肝损伤的保护效果.方法 实验分为4个预防组和2个治疗组,其中每组又分为阴性对照组、溶剂对照组（玉米油）、CCl4模型组、大蒜油低（25mg/kg）、中（50mg/kg）、高（100mg/kg）剂量组,每组15只雄性昆明种小鼠.预防组给药方案为预防1组,提前灌胃大蒜油30 d;预防2组,提前灌胃大蒜油5d,均为1次/d（10ml/kg）,末次给大蒜油后4 h灌胃1次CCl4（80mg/kg,10ml/kg）;预防3组,提前2 h灌胃1次大蒜油;预防4组,灌胃CCl4的同时给予1次大蒜油.治疗1组为灌胃CCl4后2 h灌胃1次大蒜油,治疗2组为灌胃CCl4后给予5 d大蒜油（1次/d,首次给药时间为CCl4染毒后2h）.溶剂对照组灌胃等体积的玉米油.模型组灌胃1次CCl4给药时间与各大蒜油组相同.末次给药（CCl4或大蒜油）后24 h,取血测定各组小鼠血清中丙氨酸转氨酶（ALT）和天冬氨酸转氨酶（AST）的活力,取肝脏,称重,并计算肝脏系数,同时取肝左叶进行病理组织学检查.结果 与阴性对照组相比,CCl4模型组肝脏系数明显增加,血清中ALT、AST活力明显升高,差异有统计学意义（P〈0.01）.与CCl4模型组相比,预防组各组的大蒜油低、中、高剂量组肝脏系数明显减小,血清中ALIT、AST活力明显降低,差异有统计学意义（P〈0.01）,并有剂量-反应关系;同时大蒜油可有效减轻CCl4引起的细胞坏死、气球样变等肝细胞损伤.不同预防组大蒜油的保护效果相比,预防3组效果较好.但治疗1组各项指标无明显改善,治疗2组已基本恢复正常.结论 大蒜油能预防CCl4引起的急性肝损伤,并且提前2 h给药预防效果较好.

Objective To observe and compare the protective effect of garlic oil against carbon tetra-chloride （CCl4）-induced acute liver injury.Methods The experiments include 4 preventive groups and 2 therapeutic groups. In every preventive and therapeutic group, the mice were randomized into 6 groups with 15 each, including one negative control group, one solvent control group, one CC1_4 model group and 3 garlic oil groups （25, 50, and 100 mg/kg body weight）. Before given a single gavage of CCl4（80 mg/kg）, the mice were pretreated with garlic oil by gavage in preventive group 1 （30 days, once daily）, preventive group 2 （5 days, once daily）, preventive group 3 （ahead of 2 h, once）, preventive group 4 （immediately, once） or the vehicle （corn oil, 10 ml/kg） in solvent control group. In therapeutic groups, the mice were gavaged garlic oil 2 h （once, in therapeutic 1） or for 5 days （once daily, in therapeutic 2） after administration CCl4. After 24 h of the last administration, blood was collected and centrifuged at 2500 r/min at 4？for 10 min, and serum was removed to measure ALT and AST activities. The liver was dissected, weighed to calculate the liver coefficient （relative liver weight）. At the same time, the liver samples were studied by histological examinations.Results Compared with negative group, the liver coefficient and the activities of ALT and AST in serum of model group were increased remarkably （P〈0.01）. Compared with CCl4 model group, the liver coefficient and the activities of ALT and AST in serum were decreased significantly （P〈0.01） by garlic oil dose-dependently in each preventive group. Simultaneously, histological assessment showed that garlic oil effectively alleviated hepatocyte injuries induced by CCl4. Comparing the preventive effects of garlic oil in every group, it was better in preventive group 3 than others. However, all indexes and histological examinations in therapeutic group 1 did not show the difference with those of CCl4 model group. In therapeutic group 2, all indexes recovered after 5 d of CCl4 administration. Conclusions Garlic oil can prevent acute liver injury induced by CCl4 and the effect is better in ahead of 2 h group than others.