摘要
目的:探讨小鼠腹腔内注射槲寄生凝集素对腹腔巨噬细胞功能的影响。方法:16只小鼠分为腹腔内给药组和空白组,分别取腹腔内巨噬细胞并计数,采用ELISA法测定TNF-α表达情况,Griess法测定NO表达,MTT法检测腹腔内巨噬细胞对肠癌HCT116细胞的杀伤作用。结果:腹腔内给槲寄生凝集素后可明显增加腹腔内巨噬细胞数,并增加TNF-α和NO的表达水平;激活的巨噬细胞对HCT116细胞的杀伤作用增强,其杀伤作用与TNF-α和NO表达时间一致。结论:腹腔内注射槲寄生凝集素可激活腹腔内巨噬细胞,激活的巨噬细胞对HCT116细胞有杀伤作用。
AIM: To investigate the effect of peritoneal macrophages in mice on tumoricidal activity against colon cancer HCT116, following intraperitoneal injection of Mistletoe Lectins. METHODS: 16 mice were divided into control group and intraperitoneal chemotherapy group. The number of peritoneal macrophages was counted by trypan blue exclusion. The amount of TNF-α and NO expressed by peritoneal macrophages was measured by ELISA and Griess, respectively. The cytotoxity of peritoneal macrophages supernatant against HCT116 was evaluated by MTT assay. RESULTS: It was found that the amount of peritoneal macrophages was increased, the expressions of TNF-α and NO were increased and the cytotoxicity against HCT116 in vitro was increased after intraperitoneal injection of Mistletoe Lectins. CONCLUSION: Intraperitoneal injection of Mistletoe Lectins can activate the peritoneal macrophages in mice and enhance the cytotoxity of the supernatant against HCT116 in vitro.
出处
《中国临床药理学与治疗学》
CAS
CSCD
2010年第2期126-129,共4页
Chinese Journal of Clinical Pharmacology and Therapeutics
基金
浙江省自然基金项目(Y206243)
宁波市博士基金项目(2005A610020)
宁波市医学科研项目(200530)
关键词
结直肠肿瘤
槲寄生凝集素
巨噬细胞
腹腔内化疗
Colorectal neoplasm
Mistletoe Lectins
Macrophage
Intraperitoneal chemotherapy