过氧化氢与亚硝酸降解猪肺肝素的技术比较分析

Research on the different technical condition of natural low molecular weight heparin preparation from animal by-products

目的:研究用猪肺肝素制备低分子肝素的技术方法。方法:以猪肺提取的肝素为原料,分别用过氧化氢、亚硝酸等方法降解猪肺肝素,以制备低分子肝素。通过单因素及多因素组合实验比较并优化出最佳工艺方法。结果:采用亚硝酸降解猪肺肝素,得到的低分子肝素平均分子量为(4600±95)u,分子量小于8000u的比率为(89.95±1.2)%,回收率为(80±2.3)%;采用过氧化氢降解猪肺肝素,得到的低分子肝素平均分子量为(3800±65)u,分子量小于8000u的含量为(80±1.2)%,回收率为(73±0.6)%。结论:采用亚硝酸降解猪肺肝素制取低分子肝素,产率较高,但采用过氧化氢降解猪肺肝素,得到的低分子肝素分子量小于亚硝酸制备法,但回收率低于亚硝酸制备法。结论:工艺方法可行。

Objective：Research on the different technical condition of low molecular weight heparin preparation from porcine lung.Method：Low molecular weight heparin was prepared from porcine lung purified heparin with nitrous acid degradation and hydrogen dioxide degradation.Optimize the parameter with single factor and combination experimentation.Results：The optimized reaction conditions were as following：reaction time 4 h,temperature 25 ℃,the concentration of nitrite 0.6%,pH2.6.Under these conditions,the average molecular weight of low molecular weight heparin was（4600±95） u,the content of LMWH which molecular weight was lower than 8000 u was（89.95±1.2）%,and the product recovery rate reached（80±2.3）%.Conclusion：It is feasible to prepare LMWH from porcine lung by using nitrite acid degradation,and it can obtain a higher yield of LMWH.