卡巴胆碱对脓毒症大鼠脏器促炎因子水平及功能损害的影响

Effects of carbachol on the visceral proinflammatory factors and dysfunction in rats with sepsis

目的研究卡巴胆碱(胆碱能受体激动剂)对脓毒症模型的抗炎和器官保护作用。方法♂大鼠128只,用盲肠结扎穿孔术,制备脓毒症模型。随机分为卡巴胆碱组和脓毒症组,分别静注卡巴胆碱10μg.kg-1或等量生理盐水。观察12,24h病死率、肝、肾、心功能指标、促炎症因子水平和组织含水率。结果卡巴胆碱组12,24h病死率分别为25%,50%,显著低于脓毒症组的37.5%,75%。肝、肾、心组织促炎因子、一氧化氮含量、髓过氧化物活性以及脏器功能指标和组织含水量,卡巴胆碱组均显著低于脓毒症组。结论卡巴胆碱能改善脓毒症大鼠脏器功能,降低早期病死率,其机制可能与其抑制促炎症因子水平、减轻脏器组织炎症和水肿有关。

Objective To investigate effects of carbachol on visceral proinflammatory factors and dysfunction in rats with sepsis. Method One hundred and twenty eight Sprague -dawley rats were projected to a cecal ligation and puncture （CLP） surgery, and divided randomly into two groups： sepsis group and sepsis with carbacholtreatment group （carbachol group）. Carbachol （10 ug . kg-1） or saline （sepsis group） was injected separately into penis vein immediately after CLP. The mortality rates of 12 h and 24 h after CLP, the levels of proinflammatory factors, the functional parameters and the rates of tissue water content of heart, liver and kidney were examed. Results The mortality rates of 12 h and 24 h of carbachol group were 25% and 50% respectively; all significantly lower than those of sepsis group 37.5% and 75%. The levels proinflammatory factor, nitrogen monoxide （ NO ）, myeloperoxidase （ MPO）, as well as the functional parameters and the rates of tissue water content in heart, liver and kidney were significantly lower in carbachol group than those in sepsis group. Conclusion The results indicated that carbochol apparently improve visceral dysfunction and lower mortality rates with its mechanism possibly related to inhibiting the levels of proinflammatory factor and attenuating visceral inflammation and edema in rats with sepsis.